Shanghai Sujing Industrial Co., Ltd. is one of the companies in the purification equipment industry in East China. With its solid strength, it introduces you to the introduction and explanation of the ultra-clean platform.
The advantage of the ultra-clean workbench is that it is easy to operate, comfortable, high work efficiency, short preparation time, and can be operated after 30 minutes or more. Basically, it can be used at any time. In factory production, the vaccination workload is very large, and when it is necessary to work for a long time, the ultra-clean platform is an ideal equipment. The ultra-clean workbench is driven by a three-phase motor with a power of 145-260W. The air is blown out through a “super filter” consisting of special microporous foam sheets, forming a continuous dust-free. Sterile super clean air laminar flow, the so-called "efficient special air", which removes dust, fungi and bacterial spores larger than 0.3 μm. The flow rate of the clean air is 24 to 30 m/min, which is enough to prevent the pollution caused by the nearby air, and the flow rate does not hinder the burning and disinfection of the device by the alcohol lamp or the Bunsen burner. The staff is operated under such sterile conditions to keep the sterile material uncontaminated during the transfer inoculation process. However, in the event of a power outage in the middle of the operation, materials exposed to unfiltered air will not be immune to contamination. At this time, the work should be completed quickly, and the bottle should be marked. If the material inside is in the stage of proliferation, it will not be used for proliferation and then transferred to rooting culture. If it is a general production material, it can be discarded because it is extremely rich. If it is in the rooting process, it can be left for later planting.
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The ultra-clean power supply adopts three-phase four-wire system, one of which has a zero line, which is connected to the machine casing and should be connected to the ground line. The other three lines are phase lines, and the working voltage is 380V. There is a certain order in the three-wire access circuit. If the wire is connected incorrectly, the wind is reversed. At this time, the sound is normal or slightly abnormal. The front of the clean bench is windless (can be observed with alcohol lamp flame, it should not be tested for a long time). To cut off the power supply in time, just replace the wire ends of any two of them and connect them to solve the problem. If the three-phase line is only connected to two phases, or one of the three phases is in poor contact, the machine sound is abnormal. You should immediately cut off the power supply and carefully repair it, otherwise the motor will be burnt. These common senses should be explained to the staff when they start using the ultra-clean platform, exempting accidents and losses that should not be caused.
The air inlet of the ultra-clean workbench is located at the back or the front of the front. The metal mesh cover has a common foam plastic sheet or non-woven fabric to block large particles of dust. It should be inspected and removed frequently. If the foam is found to be aging, it should be replaced in time. In addition to the air inlet, if there is a leaking pore, it should be tightly sealed, such as tape, cotton, glued paper, etc. The metal mesh cover on the front of the workbench is a super filter. The super filter can also be replaced. If it is used for a long time, the dust particles are blocked, the wind speed is reduced, and when the aseptic operation cannot be guaranteed, it can be replaced with a new one.
The length of service life of the clean bench is related to the cleanliness of the air. In the temperate zone, the ultra-clean bench can be used in general laboratories. However, in tropical or subtropical regions, due to the high level of pollen or dusty areas in the atmosphere, the ultra-clean bench should be placed in a good indoor room with double doors. use. Under no circumstances should the inlet hood of the clean bench be placed against an open door or window to avoid affecting the life of the filter.
The sterile room should be periodically dusted and disinfected with 70% alcohol or 0.5% phenol spray, wiped with 2% chlorhexidine and utensils (70% alcohol is also available), and formalin (40% formaldehyde) plus a small amount of permanganic acid. Potassium is periodically sealed and fumigation, combined with UV sterilization lamps (opening for more than 15 minutes each time), etc., so that the sterile room is always kept highly sterilized. The inside of the inoculation box should also be equipped with an ultraviolet lamp. It should be sterilized by turning on the lamp for more than 15 minutes before use. However, it is still bacteriay where it is not exposed. When the ultraviolet lamp is turned on for a long time, it can excite oxygen molecules in the air to form ozone molecules. This gas component has a strong bactericidal effect and can sterilize the corners where the ultraviolet rays are not directly irradiated. Since ozone is harmful to health, the UV lamp should be turned off before entering the operation, and it can be inserted after more than ten minutes.
On the clean bench can hoisting ultraviolet lamp, it is to be provided outside the lamp covers, lighting lamps are arranged in parallel and staggered, so that when the work does not interfere with illumination. If the UV lamp is placed inside the lighting cover (glass plate), this is useless because the ultraviolet light cannot penetrate the glass, and its lamp is made of quartz glass instead of silicate glass.

Instructions
The small environment in the clean bench can be sterilized by UV lamps, and the high-efficiency filter blast system creates a more stable sterile environment inside the table. The activities of people at work should minimize the air circulation system in the station.
Main content:
1. Purpose To standardize the operation and maintenance of the purification workbench to ensure the normal operation of the instrument.
2. Scope of application: This test method is applicable to the operation and maintenance management of the purification workbench in microbiological examination.
3. Inspector responsibility: responsible for the operation and maintenance management of this instrument
4. Operation and maintenance procedures
4.1 Operating procedures
4.1.1 When using the workbench, it should be turned on 30 minutes in advance, and the ultraviolet germicidal lamp should be turned on at the same time to process the microorganisms accumulated on the surface of the operation area. After 30 minutes, the germicidal lamp is turned off (the fluorescent lamp is turned on), and the fan is started.
4.1.2 For newly installed or long-term unused workbench, it is necessary to clean the workbench and surrounding ring mirror with an ultra-static vacuum cleaner or a tool that does not produce fibers before use, and then use drug sterilization or ultraviolet light. Sterilization is carried out by sterilization.
4.1.3 Unnecessary items are not allowed to be stored in the operation area, and the clean airflow pattern in the work area is not disturbed.
4.1.4 Try to avoid the action of the airflow pattern that is obviously disturbed in the operation area.
4.1.5 The operating temperature of the operating area should not exceed 60 °C.
4.2 Maintenance procedures and maintenance methods
4.2.1 According to the cleanliness of the environment, the coarse filter cloth (polyester non-woven fabric) can be removed or cleaned or replaced at regular intervals (generally 2 to 3 months).
4.2.2 Periodically (usually one week) sterilize the environment around the environment, and often wipe the surface of the ultraviolet germicidal lamp with gauze dipped with organic solvents such as alcohol or acetone to keep the surface clean, otherwise it will affect the sterilization effect.
4.2.3 When the fan voltage is increased and the wind speed cannot reach 0.32m/s, the high efficiency air filter must be replaced.
4.2.4 When replacing the filter, open the top cover. When replacing, pay attention to the arrow mark on the filter. The arrow points to the laminar air flow direction.
4.2.5 After replacing the high-efficiency filter, apply the dust particle counter around the frame to seal well, adjust the fan voltage, keep the average wind speed in the operating area within the range of 0.32-0.48m/s, and check with the Y09-4 dust particle counter. degree
Use management
1 Purpose: To maintain the biological cleanliness of the barrier system, to protect the safety of workers, to ensure the normal operation of the unit, and to minimize various variables that can affect scientific research results.
2 Principle: The ultra-clean workbench in the barrier system can only be used for the operation of animal models, the daily operation of transgenic mice.
3 Management:
(1) Each time the ultra-clean workbench is used, the experimenter should first turn on the UV lamp on the ultra-clean workbench and use it after 30 minutes of UV irradiation.
(2) Turn on the working power of the ultra-clean workbench, turn off the UV lamp, and spray the disinfection work surface with 75% alcohol or 0.5% peracetic acid.
(3) During the entire experimental procedure, the laboratory personnel shall operate in accordance with the aseptic operating procedures.
(4) After the end of the experiment, wipe the work surface with disinfectant, turn off the working power, and turn on the UV light for 15 minutes.
(5) In case of failure of the unit, the laboratory animal room should be notified immediately, and the professional should continue to use it after passing the inspection.
(6) The experimenter should pay attention to keep the room clean and tidy.
(7) The filter material of the ultra-clean workbench is replaced every 2 years, and the replacement record is made.
Routine maintenance
The ultra-clean workbench is a relatively sophisticated electrical device that is very important for regular maintenance and maintenance.
First of all, we must keep the indoors dry and clean. The humid air will not only rust the manufacturing materials, but also affect the normal operation of the electrical circuit. The humid air is also conducive to the growth of bacteria and mold. A clean environment also extends the life of the filter plate.
In addition, regular cleaning of equipment is an important part of normal use. Cleaning should include routine cleaning before and after use and regular fumigation. When fumigation, all gaps should be completely sealed. Cleaning should include routine cleaning before and after use and regular fumigation. When fumigation, all gaps should be completely sealed. For example, the type of ultra-clean workbench with movable baffle cover at the operation port can be sealed with plastic film.
The filter plates and UV germicidal lamps of the ultra-clean workbench have a calibrated service life and should be replaced on schedule.
Operating procedure
1. Preparation of the ultra-clean workbench before use
(1) Turn on the ultra-clean workbench fan to clean the environment inside the station. Turn on the work light to reach the working illumination.
(2) Check that all kinds of sterilized feeding and experimental items prepared in the ultra-clean work station are complete. If you need to replace the cage box. Check the preparation of the sterilization cage and use a stainless steel cart to move the ultra-clean platform to the door.
(3) Check the spray effect of the sprayer and whether the liquid used for wiping the inside and outside of the clean bench has been configured.
(4) Open the exhaust system in the room. Eliminate the odorous gas that escapes when the cage is opened.
(5) Check the various head indicators of the IVC unit and visualize the animals in each cage to estimate the operation of the IVC.
(6) Record the main environmental parameters affecting the reproduction, growth and development of experimental animals, such as temperature, relative humidity, daily temperature difference, and pressure difference between the inside and outside of the cage.
2. Ultra-clean workbench operation procedures and specifications
(1) Personnel bring disposable masks, hats and medical latex gloves. .
(2) Use both hands to gently lift the outer end of the IVC cage, move it out of the cage along the cage, and place it on the stainless steel trolley next to the ultra-clean workbench.
(3) Spray the outer surface of the glove and the outside of the IVC cage with the liquid sprayer, disinfect and sterilize the surface of the dust, and prevent it from being blown by the laminar gas when entering the clean bench. You can also use the gloved hands to immerse in the liquid medicine container, pick up and wring the liquid medicine towel, dry the outer droplets of the glove, and wipe the outer appearance of the cage.
(4) Open the ultra-clean workbench and move the cage to the ultra-clean workbench.
(5) Appropriately pull down the ultra-clean workbench sliding door. Open the fastening tab between the bottom of the IVC cage lid and open the cage. Set aside on one side.

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