There are many routes of administration in the experimental animal model administration - but most of them are administered systemically, which not only causes waste of valuable drugs, but also causes unnecessary systemic effects.

Deficiencies in the usual methods of administration for the treatment of kidney disease models:

1, tail vein injection: tail vein injection can not lock the target organ, imaging aberration

2, renal parenchymal injection: stem cell transfer rate is not high

3, kidney subcapsular injection: poor drug absorption, uneven stem cell transfer

4, renal artery injection: renal artery direct puncture injection is difficult to stop bleeding and renal artery arterial injury caused by renal artery thrombosis and other causes of experimental kidney hypoperfusion, and even loss of blood supply, leading to experimental failure

therefore. According to the cardiovascular anatomy of rats, the method of injecting drugs and stem cells into the renal artery under the direct view of the left carotid artery was successfully established. It is simple and easy, the operation time is short, and the success rate is high. Features: long-distance intubation, zero-distance administration.

Let's take a look at the vascular anatomy from imaging....

The above picture is provided by Bayer Perry Liu, Zhao Guifeng logo

Preparation of special materials

PE10 catheter: outer diameter 0.5MM inner diameter 0.3MM tip cut into 75° oblique angle, the peak is slightly bent inward.

Surgical methods:

Abdominal opening

1cm below the xiphoid xiphoid to the tail skin 6cm routine skin disinfection, spread towel exhibition, layering the incision skin and abdominal muscle along the midline of the abdomen, open the window 2cm saline warm gauze to push the intestines open, fully exposed the abdomen The artery is from the diaphragm to the left renal artery bifurcation.

Neck opening

The left common carotid artery was exposed, the distal end of the distal end of the heart was permanently ligated, the traction line was left, the proximal end was threaded alternately, and the carotid artery was obliquely cut into a small opening. The PE10 catheter was inserted into the proximal end and the ligature was proximally ligated to the proximal end. The catheter and the left common carotid artery are slightly ligated to ensure that no blood leakage is required at the entrance of the vascular catheter. The catheter enters the abdominal aorta through the left carotid artery-aortic arch descending-thoracic aorta.

Catheter introduction into the renal artery

Use a cotton swab to gently press the renal artery, the abdominal aorta bifurcation, confirm that the tube is in the abdominal aorta, gently pull the left renal artery to the right until parallel with the abdominal aorta, adjust the catheter tip to insert the left renal aorta According to the experimental requirements, the catheter can be inserted into the anterior branch of the renal artery, the posterior branch, and the adrenal artery to accurately inject the drug into the target organ.

Catheter introduction route

This method can also be effectively extended to the precise administration of other target organs, reducing the damage of drugs to other organs and the loss of valuable drugs~~~

This paper has been published in 2011 in "Chinese Journal of Comparative Medicine", authors: Zhao Guifeng.

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