1. Pollution of vomiting toxins in wheat and flour

What is vomiting toxin?

Vomiting toxin, also known as deoxynivalenol (DON), was first discovered in the 1970 strain of a scab barley virus in Kagawa Prefecture, Japan, because it can cause vomiting in pigs. For vomiting toxins. Because of their high cytotoxicity and immunosuppressive properties, they pose a threat to human and animal health, especially to immune function. Depending on the dose of DON and the exposure time, it can cause immunosuppression or immune stimulation. When people ingest food contaminated with DON, it can cause acute poisoning symptoms such as anorexia, vomiting, diarrhea, fever, unstable standing, unresponsiveness, and severe damage to the hematopoietic system. In 1998, vomiting toxins were classified as Category 3 carcinogens in an evaluation report published by the International Agency for Research on Cancer.

Vomiting toxin production and its natural distribution

It is mainly produced by Fusarium graminearum, Fusarium oxysporum, Fusarium oxysporum, Fusarium oxysporum, Fusarium oxysporum, Fusarium oxysporum and other Fusarium. Toxigenic strains of vomiting toxin are suitable for growth under cool and humid conditions and are widely found in barley, wheat, corn and oats. Because the proportion of grain in the traditional Chinese eating habits is much higher than that in the West, the harm of vomiting toxins is more prominent. Therefore, the problem of pollution caused by vomiting toxins has attracted more and more attention.

Vomiting toxins in wheat and flour and control measures

The vomiting toxin DON in wheat products: vomiting toxin is mainly distributed in the cortex of wheat grains. After the initial processing of wheat, the toxin is mainly transferred to the bran, so the bran becomes the most easily vomited toxin in the wheat primary processing product. Contaminated matrix; although the vomiting toxin DON content in flour is low, it has a wide range of pollution.

2. National Residue Limits for Vomiting Toxins in Wheat and Flour

Many countries and regions have established DON limit standards based on grain type and processing. For example, the DON limit for the EU is 200-1750μg/kg, 1000μg/kg for the United States, 600-2000μg/kg for Canada, and 1100μg/kg for Japan. In 2015, the Codex Alimentarius Commission (CAC) first issued the DON limit standard, which stipulates that the limit of DON in unprocessed grains is 2000 μg/kg, the limit in cereal products is 1000 μg/kg, and the limit in grain-based infant food is 200 μg/kg.

Third, Shanghai fly test biological wheat and flour vomiting toxin rapid quantitative detection program - -8min rapid quantification

Shanghai Feishen Bio is based on the world's leading fluorescence quantitative POCT technology platform. It is the first in China to introduce a vomiting toxin fluorescence quantitative test strip. This product can quickly and quantitatively detect the residual content of vomiting toxin in wheat and flour at 8 min. The treatment is simple (only 8min), the detection operation is simple, and the fluorescence immunoassay analyzer reading is adopted. The result is accurate and reliable, and can be printed on site. The accuracy is in accordance with the detection result of the HPLC method, and is suitable for various wheat and flour processing enterprises and testing institutions.

3.1. Vomiting toxin fluorescence quantitative test strip performance

Detection sensitivity: 25μg/kg;

Quantitative linear range: 50μg/kg - 5000.0μg/kg;

Sample preparation time: 7min;

Detection time: 8min;

Accuracy: recovery rate is 80%-125%;

Specificity: no cross-reactivity with other mycotoxins at a concentration of 1000 μg/kg;

3.2. Sample preparation process

1. Crushing (pulverization of wheat samples, direct weighing of flour);

2. Oscillation extraction (5min);

3. Centrifugation (2min);

3.3. Detection operation process

1, dilution;

2. Adding reaction (8min);

3, reading, print test report;

3.4. Results interpretation and output

The portable jade vomiting toxin detector is used to make the readings more accurate and objective, and avoid artificial misjudgment.

The test result will be presented on the liquid crystal display of the fluorescence reader. At the same time, the paper test report can be obtained by pressing the print button. In addition, after the WIFI data upload function of the instrument is turned on, the relevant data information will be automatically uploaded to the “Food Safety Traceability Management”. Cloud platform for easy traceability and quality management.

3.5. Shanghai fly test biomycotoxin series fluorescent quantitative test strip product highlights

Rapid quantification of 8min : The characteristics of rapid detection of colloidal gold, quantitative detection by enzyme-linked immunosorbent assay, and accurate detection of chromatographic mass spectrometry are combined to achieve rapid and accurate quantitative detection of mycotoxins within 8 min. The test results can be printed on site;

Built-in quantitative standard curve : The built-in standard curve of the instrument can be used to make the standard curve without testing, which saves the cost, avoids the contact of the operator with mycotoxins, and protects the safety of the operator;

On-the-go inspection : There is no requirement for the sample size to be tested, and it is possible to carry out single or small samples at the same time, or to test a large number of samples at the same time, and to realize on-site inspection;

The process is simple : the sample preparation is simple, the operation is simple, and there is no need to adjust any pH of the test sample extract;

Low configuration requirements : low requirements for supporting equipment and personnel. The minimum instrument configuration requires only one balance, one 100ul and 1ml pipettes, and the operator can be mastered with only short-term training;

Remote network support : The instrument can automatically perform standard curve reading, software upgrade, problem diagnosis, quality control, etc. through the network;

Cost-effective : the price of the flower colloidal gold test strip, the quality and results of the chromatographic mass spectrometry, and the saving of manpower and material resources, greatly reducing the cost of testing;

3.6. Shanghai fly test biomycotoxin fluorescence quantitative test strip performance

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