The mice are more docile and generally do not bite, and are easier to grasp and fix. Usually use the right hand to lift the mouse tail and place it on the squirrel cage or other rough surface. When the mouse is struggling to crawl forward, use your left thumb and forefinger to pinch the ears and neck skin, and place the mouse in the left hand. The palm, the ring finger and the little finger clip the back skin and tail to completely fix the mouse. In some extraordinary experiments, such as tail vein injection, special fixation devices can be used for fixation , such as tail vein injection frames or thick glass tubes. If surgery or cardiac blood collection is required , anesthesia should be performed first . If an anatomical experiment is performed , it must be performed without pain.

Or use this method:

(1) The mouse should be fixed, a cage should be made, and the front should be ventilated. It is best to have a baffle in the middle so that the mouse can not retreat. A mouth is opened behind the bobbin, and the tail comes out from here, so that it is firmly fixed;

(2) Soak the tail with a little hot water for a few minutes before injection, which is good for injection;

(3) Do it first and then do not start from the end of the tail.

(4) After entering the blood vessel, focus on maintaining stability, and the needle tip can easily pierce the blood vessel. As for whether you can wear it, how do you feel personally? More practice, it must be grasped quickly, not difficult, absolutely not difficult! I practiced more than 10 mice and I was more skilled.

2. wiped with alcohol cotton tail, vasodilator; or hot water, or warmed by hot towels, venous dilation; choose the appropriate needle as fine as possible; place closer to the injection in the tail section, where relatively large blood vessels . Wipe with alcohol or hot water. When wiping, pull the tail on the table. The state of injection is whitening of the tail, and two red veins are clearly visible on both sides of the white tailbone.

3. Fix the tail of the mouse with the index finger , middle finger , ring finger and thumb of the left hand; Manipulation: Hold 0.1 ml of the front of the 1 ml syringe. The little finger of the right hand is placed on the thumb of the left hand holding the tail of the mouse. Press the hand to insert the needle; look at the bevel in front of the needle tip and have 3/4 ( critical ) . If the blood vessel is full, enter 1/2 , enter, stop, pick the needle, and insert the needle. Swing gently from side to side, such as movable, can be injected. The principle is: completely fix the hand shape you first scored and repeat it every time. 4. Injection : When the injection is done, the left hand is tailed, so that the tail is close to the table, and the tail and the edge of the table are close to the turning point for the needle insertion. Generally, the needle is inserted at 1/4 or 1/3 of the tail tip, where the skin is thinner. The blood vessels are clear and the needle is easy to insert. When the needle is inserted, the needle is parallel to the table top, the needle tip is slightly downward, and the needle is inserted slightly from the middle finger and the ring finger to the thumb. Once it enters, the needle should be slightly picked up, the needle enters along the blood vessel, and the needle can be observed by the naked eye. If the needle advances in the blood vessel, it is obvious that the needle is smooth and has no resistance. If the needle is not in the blood vessel, the needle has a resistance and a feeling of blocking. Do not insert the needle too deeply. Immediately after the needle enters the skin, the needle is slightly upward, and the needle is inserted in parallel. When the needle is inserted, there is a feeling of falling. When there is no resistance when pushing the liquid, it means success! If a pimple appears at the needle, it means that it is pushed under the skin. The needle is preferably located at 1/3-1/2 of the tail, or even lower. If you fail this time, you can come back in the past. Don't fight at the highest point right away, because you have to play so many days. Be prepared for the future. The blood vessels can be dilated with hot water, alcohol, and infrared light. Can be used as appropriate. All in all, the most important thing is that " practice makes perfect. " You'd better practice it first or ask the experts to help. Otherwise, it will be very difficult to fight in the last few days.

2. Injection: After the needle enters, remember that the hand should not tremble, because the blood vessel wall is very thin and easy to pierce. When pushing the medicine, push it slowly. If the needle is successful, the drug is smooth and has no resistance; if there is resistance when pushing the drug, it means that the needle is not in the blood vessel, and it must be pulled out in time to re-enter the needle. Generally, the 4th or 4th half needle is used. It is best to use a 2 ml disposable syringe that is vaccinated. Injection of the tail vein is not difficult, mainly a feeling. If you use ten rats to practice your hand, you will find the feeling. Keep your heart steady, your hands steady, and be patient.

5. Test whether the needle is in the vein by seeing whether there is blood return ;

6. Injection . Do the above steps to ensure successful injection !
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Note:

(1) If you are doing cell transplantation, you should try to break up the cells, otherwise it will easily cause embolism!

(2) Exercise: I suggest you use blue ink to practice your hand first, because novices often don't see if they are subcutaneous or veins. In fact, they can only be cooked. It is recommended to enter the needle at the bottom 1/3 of the tail, but I think 1/2 The accuracy is better, the personal experience needle can be inserted deeper to prevent accidental swinging and puncture the vein and injected into the skin. Many times, you feel that it is subcutaneous under the blood vessel, for this reason.

Or use: often the tail vein injection of placenta orchid, relatively easy, the vein on the side of the thicker one, can be a container, we use a 50ml centrifuge tube in front of an opening, the back of the screw cover to make a hole, the mouse It is relatively easy to go in, and the mouse is automatically placed in the tube, and the tail is pierced from the hole of the cover, which is extremely convenient to operate. Shaving, hot with hot water, blood vessels can rise up, the specific feelings have to practice their own grasp, but it is easy, there are 3-4 can be grasped.

(3) Regularity: The smaller the animal, the better the operation, the mouse is easier than the rat, the young rat is easier than the adult rat; the best for the mouse is the tail vein injection, so the damage is minimal; the adult rat can't be selected. Intravenous injection is relatively easy; the most important point is that you must practice more. The feeling of entering the needle must be well understood. It is common sense that practice makes perfect.

Skillful skills are important, and precision syringes are an essential part of getting good data . HAMILTON micro-injectors are the world's top brands. Using high-quality materials and skilled manufacturing processes, each product has high performance, high precision and unique features to meet customer requirements in the field of fluid precision measurement. The accuracy of the Hamilton syringe is within ±1% of the nominal value and the quality of the product is certified to EN ISO 9001/EN 46001 .

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