Introduction to CCK-8 Test Kit

WST-8 is an upgraded product of MTT, which can be reduced by dehydrogenase in mitochondria to form a highly water-soluble orange-yellow formazan product (formazan) in the presence of an electron coupling reagent. The depth of color is directly proportional to the proliferation of cells and inversely proportional to cytotoxicity. The OD value was measured at a wavelength of 450 mM using a microplate reader, indirectly reflecting the number of viable cells.

CCK-8 Test Kit - Product Advantages:

CCK-8 Test Kit - provides a high sensitivity, easy to operate, safe to use cell proliferation and toxicity test method, compared with the traditional MTT test has obvious advantages:

The CCK-8 method is used for the detection of cell proliferation induced by cytokines and the like, and can also be used for the detection of cytotoxicity induced by toxic agents such as anticancer drugs, or the detection of cell growth inhibition by some drugs, which is related to cell activity and proliferation. The experiment is a highly sensitive, non-invasive colorimetric assay.

The CCK-8 method is non-toxic to cells, and the optimal measurement time can be selected by multiple measurements. Compared with the MTT method, the linear range is wider and the sensitivity is higher.

CCK-8 solution can be directly added to the cell sample without preparation. It is ready to use and is more stable than MTT. The experimental results are reproducible and suitable for large-scale, high-throughput sample detection.

The formazan produced by the MTT experiment is not water-soluble and needs to be dissolved by an organic solvent such as DMSO; and the formazan produced by the method is water-soluble, which not only eliminates the dissolution step, but also reduces the error caused by the operation step.

CCK-8 test kit storage conditions

Please receive the Abbkine brand [CCK-8] cell proliferation/toxicity test kit product and store at 0-5 °C in the dark.

How to choose the right CCK-8 test kit ?

The [CCK-8] cell proliferation/toxicity test kit currently available on the market is available in domestic or imported products. The domestically packaged CCK-8 kit has insufficient quality stability. The CCK-8 kits, which are native to domestic laboratories, have some good test results and are difficult to control in batch stability. As for the imported brands that supply CCK-8 kits, there are not many imported brands. Even Abcam, Thermo, Sigma and other brands can not provide CCK-8 kits, while other brands are far less expensive than Abbkine products.

As the flagship product of the Abbkine brand's first cell analysis product, the Cell Counting Kit (CCK-8) , Abbkine R&D engineers devoted a lot of investment to continually exploring and optimizing the best formula. Each effort is only for the researchers to be more Simple, efficient, and stable access to valuable scientific data.

The CCK-8 test kit is very easy to detect. Only one tube of CCK-8 solution containing WST-8 has been prepared in the kit, no need to perform any preparation and other operations. No isotope is required and all detection steps are performed in the same 96-well plate. It is not necessary to wash the cells, it is not necessary to collect the cells, and there is no need to take extra steps to dissolve the formazan. Can be used for the detection of large quantities of samples.

"CCK-8 Test Kit - Comprehensive Comparison of Cell Proliferation/Toxicity Detection"

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