What is sample breakage? Efficient fragmentation and homogenization of biological samples is an absolute requirement for all RNA purification processes. The fragmentation releases the RNA from the sample, while the homogenization reduces the sample viscosity and facilitates subsequent RNA purification. Why use QIAGEN 's sample crushing products? QIAGEN offers a wide range of technologies for crushing and homogenizing – from QIAshredder spin columns to the TissueRuptor and TissueLyser systems, the former is suitable for simple and rapid homogenization of cell lysates, while the latter is suitable for mechanical crushing and homogenization of harder tissues. the amount. The TissueRuptor and TissueLyser systems bring fast and efficient crushing and replace the tedious and time-consuming methods of crushing such as mortars and mortars. How does QIAGEN 's sample crushing products work? The QIAshredder is a biomacromolecular crushing system in the form of a spin column. The cell lysate is loaded onto a QIAshredder spin column and then briefly centrifuged to homogenize the lysate. The TissueRuptor is a handheld device that uses a TissueRuptor disposable probe to simultaneously break and homogenize. The probe contains a blade that rotates at very high speeds. Since the probe is disposable and transparent, the risk of cross-contamination is minimized and the sample disruption process is intuitively monitored. The use of disposable probes also saves time because there is no need to clean the probe after breaking each sample. The TissueLyser instrument is a bead mill that simultaneously breaks and homogenizes the sample by high-speed vibration of the beads in a plastic tube. With an adapter that can accommodate manifolds, the instrument can simultaneously break multiple samples – the TissueLyser LT can handle up to 12 samples, while the TissueLyser II can process up to 48 or 192 samples. Figure 1 Efficient tissue disruption: Different rat tissues were disrupted using TissueLyser LT or TissueLyser II. [A] DNA was purified from 25 mg samples using the DNeasy Blood & Tissue Kit on a QIAcube automated nucleic acid purifier. DNA yield was measured using a spectrophotometer. [B] RNA was purified from a 20 mg sample using the RNeasy Fibrous Tissue Mini Kit (skin, heart and lung) or the RNeasy Lipid Tissue Mini Kit (brain) on a QIAcube automated nucleic acid purifier. RNA production was measured using a spectrophotometer. Product ordering information: product name specification Item number QIAshredder (50) For homogenization of cell lysates 79654 TissueRuptor® For disruption of private samples 9001271 TissueRuptor Disposable Probes (25) Disposable probes for use with the TissueRuptor 990890 TissueLyser LT For disruption of up to 12 samples 85600 TissueLyser LT Adapter, 12-tube For purification of RNA from cells in 96-well format 69980 TissueLyser II For disruption of up to 48 or 192 samples 85300 TissueLyser Adapter Set 2 x 24 Adaptor set for use with the TissueLyser II; holds 48 tubes 69982 The above information comes from QIAGEN official website! Read the original: http:// Puyang Linshi Medical Supplies Co., Ltd. , https://www.linshimedicals.com
How QIAGEN products for sample crushing work