It is well known that ubiquitin (Ub) is a highly conserved small protein that can be covalently linked to the lysine residue of the target protein to form a polyubiquitin chain to function to guide protein degradation. Similar to the ubiquitination modification process, the small ubiquitin related modifier (SUMO) can also covalently modify the lysine residue of the target protein. Currently, SUMOylation has become a well-known translation of eukaryotic cells. One of the post-protein modification markers. This reversible covalent linkage between SUMO and the target protein plays an important regulatory role in nucleoplasm transport, DNA and protein binding activity, protein interactions, transcriptional regulation, DNA repair, and maintenance of genomic stability. In many human diseases such as cancer and neurodegenerative diseases, SUMO modification plays an important role in the occurrence and development of diseases. It is also the case that attracts more and more scientists from around the world to participate in SUMO. The wave of research is coming!

Figure 1 - (SUMO modification schematic)

So how to quantitatively detect the SUMO of the target protein ?

Products currently on the market are detected using affinity chromatography or WB methods. Not only are the data results unintuitive, but the accuracy is poor and cannot provide convincing results. In order to solve this problem and better serve the majority of researchers, Epigentek has carefully researched and creatively introduced a SUMO-based quantitative detection kit (patent) similar to ELISA: EpiQuik In Vivo Protein Sumoylation Assay Ultra Kit (P-8003) .

As a professional solution provider for epigenetics, Amy Jie is proud to recommend Epigentek's P-8003 kit. This kit provides a complete set of optimized reagents for the detection of cell and tissue samples from mammals including humans, mice, and rats. Using your IP-specific antibodies to your protein of interest, you can target proteins. The SUMO content is subjected to relative detection and absolute detection. For best results, we recommend the use of the matching nuclear protein extraction kit: EpiQuik Nuclear Extraction Kit (OP-0002).

The EpiQuik In Vivo Protein Sumoylation Assay Ultra Kit also has the following advantages:

• Fast response, as long as 5h
• Direct color reaction, no need for affinity chromatography or WB steps
• Flexible selection of antibodies for detection of SUMOylation of multiple proteins of interest
• The use of optimized antibodies eliminates the preparation of the detection solution, which greatly increases the sensitivity and convenience of the reaction.
• The strip-shaped reaction plate is suitable for different experimental needs: it can be manually tested or high-throughput test
• Reliable and stable reaction conditions

Figure 2. (P-8003 kit quick experimental flow chart) Figure 3. (P-8003 kit standard curve)

Epigentek Group is the world's leading developer and supplier of innovative technology and epigenetic research products. The company has developed a comprehensive product portfolio of more than 400 patented products to provide comprehensive system solutions for epigenetic research and new drug development. As the general agent of Epigentek Group's China region, Amy Jie introduced it to China as its regional agent in China and will provide customers with complete epigenetic solutions, including analytical kits for antibodies, antibodies and related Products such as inhibitors/antagonists. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line 400-6800-868 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.

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