Materials:

MDA-MB-231-Luc and HCT116-Luc cells

Mice: 2 subcutaneous HCT116-Luc tumor-bearing female BALB/c nude mice;

4 naïve female BALB/c nude mice;

Artagain black paper (Strathmore, Catalog# 445-109)

D-Luciferin Firefly, potassium salt(Sciencelight, Catalog# luc001), for in vivo imaging

Syringe (1ml) and needles (25 x 5/8" gauge)

Anesthesia (Pentobarbital Sodium)

Procedure:

A. Luciferin Preparation

1. Prepare a stock solution of luciferin at 15mg/ml in DPBS. Filter sterilize through a 0.2 um filter.

2. Prepare enough to inject 10 ul/g of body weight. Each mouse should receive 150 mg luciferin/kg body weight. (eg For a 20 g mouse, inject 200 ul to deliver 3 mg of luciferin.)

B. Preparation of tumor cells:

1. Trypsinize the cells from flasks, wash once in serum-free medium.
2. Resuspend in room temperate serum-free medium to a concentration of 1x10 ⁶ cells in 100ul. Hold at room temperature until ready for use.

C. Injections:

1. Inject mice with firefly luciferin (150 mg/kg) by intraperitoneal injection using a 25 x 5/8” gauge needle.

2. After 7-8 minutes, anesthetize mice by intraperitoneal injection of pentobarbital sodium using a 25 x 5/8" gauge needle.

3. (1) Inject tumor cells (1x10 ⁶ cells in 100ul) subcutaneously into the dorsal flank of the mouse.

(2) For the two HCT116-Luc tumor-bearing mice, no cells were inoculation to the mice.

D. Imaging

Place mice on black paper in the imaging box and image dorsally to detect tumor cells.

Materials:

MDA-MB-231-Luc and HCT116-Luc cells

Mice: 2 subcutaneous HCT116-Luc tumor-bearing female BALB/c nude mice;

4 naïve female BALB/c nude mice;

Artagain black paper (Strathmore, Catalog# 445-109)

D-Luciferin Firefly, potassium salt(Sciencelight, Catalog# luc001), for in vivo imaging

Syringe (1ml) and needles (25 x 5/8" gauge)

Anesthesia (Pentobarbital Sodium)

Procedure:

A. Luciferin Preparation

1. Prepare a stock solution of luciferin at 15mg/ml in DPBS. Filter sterilize through a 0.2 um filter.

2. Prepare enough to inject 10 ul/g of body weight. Each mouse should receive 150 mg luciferin/kg body weight. (eg For a 20 g mouse, inject 200 ul to deliver 3 mg of luciferin.)

B. Preparation of tumor cells:

1. Trypsinize the cells from flasks, wash once in serum-free medium.
2. Resuspend in room temperate serum-free medium to a concentration of 1x10 ⁶ cells in 100ul. Hold at room temperature until ready for use.

C. Injections:

1. Inject mice with firefly luciferin (150 mg/kg) by intraperitoneal injection using a 25 x 5/8” gauge needle.

2. After 7-8 minutes, anesthetize mice by intraperitoneal injection of pentobarbital sodium using a 25 x 5/8" gauge needle.

3. (1) Inject tumor cells (1x10 ⁶ cells in 100ul) subcutaneously into the dorsal flank of the mouse.

(2) For the two HCT116-Luc tumor-bearing mice, no cells were inoculation to the mice.

D. Imaging

Place mice on black paper in the imaging box and image dorsally to detect tumor cells.

Disposable Endo Fascia Closure System

Disposable minimally invasive fascial closure device is a medical device used to close the abdominal fascia layer after laparoscopic surgery. The device consists of a needle and suture that can be inserted through a small incision in the skin and used to close the layers of fascia without the need for a larger incision. The device is designed to reduce the risk of complications associated with traditional methods of fascial closure, such as wound infection, hernia and dehiscence. The single-use nature of the device also reduces the risk of cross-contamination and infection. Use of the device could shorten recovery time and improve outcomes for patients undergoing laparoscopic surgery.