CRISPR/Cas9 gene editing technology is widely used in knockout mouse experiments because of its high efficiency, easy operation and low cost. However, in the process of injecting RNAs into fertilized eggs, traditional microinjection technology needs to be used. Micro-operating systems manually inject fertilized eggs one by one, which is very time consuming. At the same time, in order to improve the success rate of gene knockout, it is necessary to obtain more fertilized eggs, and artificially designed gene targeting schemes are time-consuming and laborious. These factors increase the development time and cost of knockout mice to varying degrees. .


In order to provide customers with faster and more efficient services, the industry has set a goal of “three times the efficiency of gene knockout and half the price”, and improved from the aspects of program design, fertilized egg injection and superovulation technology. Firstly, starting from the injection of fertilized eggs, the traditional microinjection method was replaced by electroporation, and the CRISPR-Pro gene editing technology was developed on the basis of CRISPR/Cas9 technology. The technology adopts a system and supporting system which is most suitable for the fertilization of animal fertilized eggs, and special treatment is carried out on the fertilized eggs of mice developed to a special stage, and then placed in an electric field for electric shock treatment, and a large number of fertilized eggs can be electrically operated. The CRISPR-Pro technology breaks through the efficiency bottleneck of the injection process, making high-throughput production possible. In the second half of 2015, the industry's bio-application of CRISPR-Pro high-throughput electro-fertilization method, the first batch of 100 knockout mice project, the rate of survival of the fertilized egg and development into a two-cell stage is 96-98%. It is nearly three times higher than 30-35% of the microinjection method. In addition, Saiye Biotech and the well-known bioreagent company jointly developed the ovulation induction reagent. After a series of optimizations, the number of eggs supplied to the oviposited mice was increased by 2-3 times, which greatly reduced the use of the mice and increased the number of mice. Animal welfare saves costs and improves preparation efficiency. Other data also show that CRISPR-Pro electroporation eggs and ovulation induction technology have greatly improved the success rate of the project.


Secondly, Saiye Biotech has optimized the gene knockout design program and made major breakthroughs. After 11 years of development, Saiye Biotechnology has the unique advantage of having a first-class team of top international biotechnology experts, bioinformatics experts and IT technology experts. The team has successfully developed the world's first gene carrier intelligent design platform VectorBuilder. () and has served tens of thousands of laboratories around the world. In response to the time-consuming and laborious problem of artificially designed gene targeting solutions, Saiye Biotech has developed an intelligent scheme design system, the Alphaknockout Gene Targeting Expert Program System. The system draws on the deep learning mechanism of “Alpha Dog”, and uses the world’s fastest supercomputer “Tianhe No.2” to conduct large-scale calculations for six consecutive times, and the gene targeting technology to make genetic engineering mouse design process artificial. Intelligent. The most valuable thing is that Saiye Bio has successfully completed thousands of mouse and rat gene targeting projects in the past few years, and accumulated a large amount of reliable data to train our artificial intelligence system, allowing the system to carry out in-depth learning. The optimal system parameters are achieved. This is similar to the Alpha dog learning a lot of human game. Now just log in to and enter the gene name for 10 seconds to get a well-designed gene knockout protocol that greatly reduces the time-consuming cost of knockout mice.


The high-throughput electroporation technology and ovulation induction technology and the intelligent AlphaKnockout gene targeting expert program system greatly save the time cost and technical cost of the knockout mice, and the gene knockout efficiency is more than three times, the complete gene The price of knockout mice has also decreased from 59,600 to 29,800, and the goal of “increasing efficiency by 3 times and price by half” has finally been achieved. As the world's leading GM/knockout mode animal commercial technology platform, Saiye will continue to bring technological innovation to the end with technological innovation as a breakthrough.

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