1. Beef extract peptone medium (for bacterial culture) Beef extract 3g , peptone 10g , NaCl 5g , water 1000mL , pH 7.4 ~ 7.6 . 2. Gao's No. 1 medium ( for actinomycete culture ) Soluble starch 20g , KNO 3 1g , NaCl 0.5g , K 2 HPO 4 •3H 2 O 0.5g , MgSO 4 •7H 2 O0.5g , FeSO 4 •7H 2 O0.01g , water 1000mL , pH 7.4 ～ 7.6 . Note when preparing: Soluble starch should be mixed with cold water before adding to the above medium. 3. Mading Shi (Martin) medium (for separating from soil fungi) K 2 HPO 4 1g , MgSO 4 •7H 2 O0.5g , peptone 5g , glucose 10g , 1/3000 Bengal red aqueous solution 100mL , water 900mL , natural pH , 121 °C moist heat sterilization for 30min . Streptomycin ( streptomycin content 30 μg/mL) was added when the medium was melted and cooled at 55 to 60 °C . 4. Potato medium (PDA) ( for mold or yeast culture ) Potato ( peeled ) 200g , sucrose ( or glucose ) 20g , water 1000mL , the preparation method is as follows: Peel the horse ring, cut into small pieces of about 2cm 2 , boil in a 1500mL beaker for 30min , pay attention to stir with a glass rod to prevent the bottom, then filter with double gauze, add the filtrate to sugar, and then make up to 1000 mL , natural pH , mold with sucrose, yeast with glucose. 5. Clarion medium ( sucrose nitrate sodium medium ) ( for mold culture ) Sucrose 30g , NaNO 3 2g , K 2 HPO 4 1g , MgSO 4 •7H 2 O0.5g , KCl 0.5g , FeSO 4 •7H 2 O0.1g , water 1000mL , pH 7.0 ～ 7.2 . 6. Hayflik medium ( for mycoplasma culture ) Digestive bovine heart (or leachate) 1000mL, peptone 10g, NaCl 5g, agar 15g, pH7.8 ~ 8.0, dispensing bottle 70mL, 121 ℃ heat sterilization 15min, cooling to about 80 ℃, 70mL of each join The Serum 20mL , 25% fresh yeast extract 10mL , 15 acetic acid hydrazine aqueous solution 2.5mL , penicillin G potassium salt aqueous solution ( 200,000 units or more ) 0.5mL , above the mixture, pour the plate. * Note: Barium acetate is a very toxic drug and requires special attention to safe handling. 7. Maxwell (McClary) medium (Medium sodium acetate) Glucose 0.1g, KCl 0.18g , yeast extract 0.25g , sodium acetate 0.82g , agar 1. 5g , distilled water l00mL . After dissolution dispensing tube, 1l5 ℃ moist heat sterilization 15min. 8. Glucose protein hydrophobic medium ( for VP reaction and methyl red test ) Peptone 0.5g , glucose 0.5g , K 2 HPO 4 0.2g , water 100mL , pH 7.2 , 1l5 °C wet heat sterilization for 20min . 9. Peptone water culture medium ( for sputum test ) Peptone 10g , NaCl 5g , water 1000mL , pH 7.2 ~ 7.4 , 121 °C wet heat sterilization for 20min . 10. Sugar fermentation medium ( for bacterial sugar fermentation test ) Peptone 0.2g , NaCl 0.5g , K 2 HPO 4 0.02g , water 100mL , bromothymol blue (1% aqueous solution ) 0.3mL , sugar lg . Weigh the peptone and NaCl separately in hot water, adjust the pH to 7.4 , then add bromothymol blue ( dissolved with a small amount of 95% ethanol, then add water to make a 1% aqueous solution ) , add sugar, and dispense test tubes. The volume is 4 ~ 5cm high, and put into a Du's small tube (the tube mouth is down, the tube is filled with the culture solution ) . Sterilize at 115 °C for 20 min . When sterilizing, please pay attention to prolonging the boiling time, and drain the cold air as much as possible so that no air bubbles remain in the Du's small tube. Commonly used sugars, such as glucose, sucrose, mannose, maltose, lactose, galactose, etc. (the latter two sugars are often increased by 1.5%) . 11. RCM medium ( enhanced Clostridium culture medium ) , ( for anaerobic culture ) Yeast extract 3g , beef extract l0g , peptone 10g , soluble starch lg , glucose 5g, cysteine ​​hydrochloride 0.5g , NaCl 3g , NaAc 3g , water 1000mL , pH 8.5 , resazurin 3mg/L , l2l °C Sterilize for 30 minutes . 12.TYA medium ( for anaerobic culture ) Glucose 40g , beef extract 2g , yeast extract 2g , bacto-typetone 6g , ammonium acetate 3g , KH 2 PO 4 0.5g , MgSO 4 •7H 2 O0.2g , FeSO 4 •7H 2 O0.01g , water 1000mL , pH6.5 , 121 °C moist heat sterilization for 30min . 13. Corn mash medium ( for anaerobic culture ) Corn flour 65g , tap water 1000mL , mix well, cook for 10min into a paste, natural pH , 121 °C moist heat sterilization for 30min . 14. Neutral red medium ( for anaerobic culture ) Glucose 40g , tryptone 6g , yeast extract 2g , beef extract 2g , ammonium acetate 3g , KH 2 PO 4 5g , neutral red 0.2g , MgSO 4 •7H 2 O0.2g , FeSO 4 •7H 2 O0.01g, water 1000mL , pH6.2 , 121 °C wet heat sterilization for 30min . 15.CaCO 3 gelatin wort medium ( for anaerobic culture ) Wort (6 Baume) 1000mL, water 1000mL, CaCO 3 10g, gelatin 10g, pH6.8,121 ℃ moist heat sterilization 30min. 16.BCG milk medium ( for lactic acid fermentation ) (A) Solution: Skim milk powder 100g, 500 mL of water, was added 1.6% bromocresol green (BCG) in ethanol 1mL, 80 ℃ sterilized 20min. (B) Solution: 10 g of yeast extract , 500 mL of water , 20 g of agar , pH 6.8, and 121 ° C for 20 min . Mix the (A) and (B) solutions evenly under aseptic conditions and then pour the plate. 17. Lactic acid bacteria medium ( for lactic acid fermentation ) Beef extract 5g , yeast extract 5g , peptone 10g , glucose 10g , lactose 5g , NaCl 5g , water 1000mL , pH6.8 , 121 °C moist heat sterilization for 20min . 18. Alcohol fermentation medium ( for alcohol fermentation ) Sucrose 10g, MgSO 4 • 7H 2 O 0.5g, NH 4 NO 3 0.5g, 20% bean sprouts 2mL , KH 2 PO 4 0.5g , water 100mL , natural pH . 19. Kosov medium ( for Leptospira culture ) Quality protein 胨 0.4g, NaCl 0.7g, KCl 0.02g, NaHCO 3 0.01g , CaCl 0.02g , KH 2 PO 4 0.09g , NaH 2 PO 4 0.48g , distilled water 500mL , sterile rabbit serum 40mL . System of law: The remaining ingredients except rabbit serum mixed and dissolved by heating, adjusted to pH 7.2, 121 ℃ heat sterilization 20min, after cooling, addition of the sterile rabbit serum, serum solution made of 8%, aliquoted tubes (5 ~ 10mL / tube), inactivated at 56 ° C water bath for 1 h and set aside. 20. Bean Sprout Medium Soybean bud 500g , add water 1000mL , boil for lh , filter to make up the water, store at 121 °C after moist heat sterilization, this is 50% bean sprouts; 10% bean sprouts juice 200mL , glucose ( or sucrose ) 50g , water 800mL , pH 7.2 ~ 7.4 . 10% bean sprout juice 200mL , sugar 50g , water 800mL , natural pH . The mold uses sucrose, and the yeast uses glucose. 21.LB (Luria-Bertani) medium ( bacterial culture, often used in molecular biology ) Double distilled water 950mL , tryptone l0g , NaCl l0g , yeast extract (bacto- yeast extract) 5g , adjust the pH value to 7.0 with 1mol / L NaOH ( about 1 mL) , add double distilled water to the total volume of 1L , 121 °C damp heat Sterilize for 30 min . Ampicillin-containing LB medium: After sterilized LB medium cooled to about 50 ℃ antibiotic was added to a final concentration of 80 ~ 100mg / L. Production process: First, peptone, beef extract, yeast extract and agar were added to 900 mL of water, dissolved by heating, and then added with K 2 PO 4 . After dissolution, the water was added to 1000 mL , and the pH was adjusted to 7.2 to 7.4 . Lactose was then added, mixed and dissolved, and sterilized by moist heat at 115 °C for 20 min . Weigh the sodium sulfite into a sterile empty test tube, dissolve it with a little sterile water, boil it in a water bath for 10 minutes , then immediately add it to 20 mL of 5% alkaline reddish ethanol solution until the deep red color turns into a pale pink until. Add all the mixture to the above sterilized medium which is still kept in the state of being melted. Immediately after mixing, pour the plate. After solidification, store the refrigerator for use. If the color changes from reddish to deep red, it can no longer be used. . 23. Lactose peptone semi-solid medium ( for the determination of coliforms in water ) Peptone 10g , beef extract 5g , yeast extract 5g , lactose 10g , agar 5g , distilled water 1000mL , pH 7.2 ~ 7.4 , divided tubes (l0mL / tube ) , moist heat sterilization at 115 °C for 20min . 24. Lactose peptone broth ( for multi-tube fermentation to detect coliforms in water ) Peptone 10g , beef extract 3g , lactose 5g , NaCl 5g , distilled water l000mL , 1.6% bromocresol purple ethanol solution lmL . Adjust the pH to 7.2 , dispense the tube (10 mL / tube ) , and put it into the inverted Du's tube, and sterilize it at l15 °C for 20 min . 25. Triple concentrated lactose peptone culture solution ( for determination of coliforms in water ) The nutrients in the lactoprotein broth were added to 1000 mL of water in a three- fold expansion . The method was the same as above, and the tubes were placed in a test tube containing inverted Du's tubules, 5 mL per tube , and sterilized by moist heat at 1 l5 °C for 20 min . 26. Eosin blue medium (EMB medium ) ( for coliform assay and bacterial transduction in water ) Peptone l0g, lactose 10g, K 2 HPO 4 2g, agar 25g, 2% / eosin Y (Eosin) aqueous solution 20mL, 0.5% methylene blue (methylene blue) solution l3mL, pH7.4. Production process: First mix the peptone, lactose, K 2 HPO 4 and agar, heat and dissolve, adjust the pH to 7.4 , heat sterilization at 1l5 °C for 20min , then add the separately sterilized eosin and methylene blue, mix thoroughly to prevent Create bubbles. Pour the plate until the medium is cooled to about 50 °C. If the medium is too hot, it will produce excessive agglomerated water, which can be stored in the refrigerator after the plate is solidified. In the bacterial transduction experiment, galactose was used instead of lactose, and the remaining components were unchanged. 27. Double broth medium ( for bacterial transduction ) Beef extract 6g , peptone 20g , NaCl 10g , water 1000mL , pH 7.4 ~ 7.6 . 29. Bean cake slant medium ( for the screening of protease-producing strains ) 100g of bean cake is added with water 5 ~ 6 times, 100mL of filter juice is boiled , KH 2 PO 4 0.1% , MgSO 4 0.05%, (NH 4 ) 2 SO 4 0.05% , soluble starch 2% , pH6 , agar 2% ～ 2.5% .   30. Casein medium ( for protease strain screening ) Prepare solution A and solution B separately . Weigh Na 2 HPO 4 • 7H 2 O1.07g . 4 g of casein , add appropriate amount of distilled water, and dissolve by heating. KH 2 PO 4 0.36 g was weighed and dissolved in water. After mixing A and B , add 0.3 mL of casein hydrolysate, add 20 g of agar , and finally make up to 1000 mL with distilled water . Preparation of casein hydrolysate: 1 g of casein was dissolved in an alkaline buffer, and 1 mL of Bacillus subtilis protease 25 mL was added to water to 100 mL , and hydrolyzed at 30 ° C for 1 h . When the medium is used for preparation, the amount is 1000 mL , and 100 mL of the above hydrolyzate is added to the medium. 31. Bacterial minimal medium ( for screening for auxotrophs ) Na 2 HPO 4 • 7H 2 O1g , MgSO 4 • 7H 2 O0.2g, glucose 5g, NaCl 5g, K 2 HPO 4 lg, water l000mL, pH7.0, 1l5 ℃ moist heat sterilization 30min. 32.YEPD medium ( for yeast protoplast fusion ) Yeast powder 10g , peptone 20g , glucose 20g , distilled water 1000mL , pH6.0 , 115 °C moist heat sterilization for 20min . 33. YEPD hypertonic medium ( for yeast protoplast fusion ) Was added 0.6mol / L of NaCL, 3% agar in YEPD medium. 34.YNB basic medium ( for yeast protoplast fusion ) 0.67% yeast nitrogen base (YNB, amino acid free, Difco), 2% glucose, 3% agar, pH 6.2 . Another recipe is: Glucose 10g (NH 4) 2 SO 4 1g, K 2 HPO 4 0.125g, KHPO 4 0.875g, KI 0.0001g, MgSO 4 • 7H 2 O0.5g, CaCl 2 • 2H 2 O0.lg, NaCl0.1g, Victoria The amount of mother liquor lmL , vitamin mother liquor lmL ( mother liquor is prepared according to routine ) , water 1000mL , pH 5.8 ~ 6.0 . 35.YNB hypertonic minimal medium ( for protoplast fusion ) Was added 0.6mol / LNaCl in YNB minimal medium. 36. Phenol red semi-solid column medium ( used to check the relationship between oxygen and bacteria growth ) Peptone 1g , glucose 10g, corn syrup 10g , agar 7g , water 1000mL , pH 7.2 . After adjusting the pH , a few drops of 1.6% phenol red solution were added until the medium became dark red, and the mixture was placed in a large test tube, and the amount was about 1/2 of the height of the test tube , and sterilized at 11.5 ° C for 20 min . Bacteria in this medium use glucose to grow acid, which makes the phenol red change from red to yellow. The bacteria growing in different parts can change the color of the corresponding part of the medium , but pay attention to the culture time is too long, the acid can spread so that it can not Correctly judge the result. Intermediate for CAS 170729-80-3 Aprepitant Intermediate,Aprepitant Api,Aprepitant Cas 30071-93-3,Trifluoromethyl Acetophenone Shandong Bolode Bio-technology Co., Ltd. , https://www.bldpharma.com
For bacterial culture:
For mold or yeast culture:
22. Complex sodium sulfite medium ( Endo's medium ) , ( for determination of coliforms in water ) peptone 10g , beef extract 5g , yeast extract 5g , agar 20g , lactose 10g , K 2 HPO 4 0.5g , anhydrous sodium sulfite 5g , 5% alkaline reddish ethanol solution 20mL , distilled water 1000mL .
28. Semi-solid agar ( for bacterial transduction ) 1 g of agar , 100 mL of water , and sterilized by damp heat at 121 °C for 30 min .
Liquid A :
B liquid:
The above various media can be formulated into a solid or semi-solid state, and only need to change the amount of agar, the former is 1.5% to 2.0% , and the latter is 0.3% to 0.8% .
Description of the preparation of 36 kinds of medium commonly used in microbiology experiments