Kit enzyme substrate Enzyme substrate 2. In the ELISA kit, DH2 is generally a colorless compound which, after enzymatic action, becomes a colored product for colorimetric determination. Commonly used hydrogen donors are O-phenylenediamine (OPD), tetramethylbiphenyl (3,3", 5,5tetramethylbenzidine, TM and ABTS[2,2"-azino-di-(3- ethylbenziazobine Sulfonate-6)]. 3. OPD oxidized product is orange-red. After acid-stopping enzyme reaction, it has the highest absorption peak at 492 nm, high sensitivity, and colorimetric convenience. It is the most commonly used substrate for HRP conjugate. OPD itself is hardly soluble in water, and OPD? 2HCL is water soluble. It has been reported that OPD has variability and should be taken care of during operation. OPD sees light and is easy to change. It is more unstable after mixing with hydrogen peroxide to form a substrate. It must be used now. In the kit, OPD and H2O2 generally divide the two components, OPD can be made into a certain amount of powder or tablet method, the tablet contains foaming cosolvent, and it is more convenient to use. 4. Hydrogen peroxide is added to the substrate buffer. The ELISA kit has a concentrate that is easy to store and is diluted with distilled water. The advanced ELISA kit is directly formulated with a protective solution containing 0.02% H2O2. It can be used as a substrate solution only after participating in OPD. TMB is treated with HRP *** The product is blue, and the visual contrast is obvious. 5. The nature of TMB is relatively stable, and it can be formulated into a solution reagent. It only needs to be mixed with H2O2 solution to form a working solution, which can be directly used as a substrate. Others, TMB has no carcinogenic benefits, so it is increasingly used in ELISA. After the enzyme reaction is stopped by HCL or H2SO4, the TMB product is yellow in blue and can be quantified in a colorimeter with an optimum absorption wavelength of 405 nm. 6. ABTS is not as sensitive as OPD and TMB, but the blank value is extremely low and is also selected for some kits. Another substrate for HRP is 3-(4-hydroxy)phenly propionic acid (HPPA). After HRP, the product is fluorescent and can be measured by a fluorometer. The benefit for ELISA is a linear programming that can broaden the quantitative determination. 7. HRP has high specificity for hydrogen receptors. The ELISA kit only acts on H2O2, peroxides of small alcohols and urea peroxide. H2O2 is the most widely used, but urea peroxide is solid, and it is safer and safer than H2O2. The kit is supplied with a urea peroxide tablet, which is dissolved in distilled water and sealed in a substrate buffer at a low temperature (2 to 8 ° C) for one year.
The Digital Video Recorder, also called the DVR/ DVR Security System/ DVR monitor/Digital Video Recorder H.264/ DVR CCTV, is the video storage device of the AHD Four in One Camera(the video capture device). The Digital Video Recorder records the video with the hard disk that we put in it, and it is a computer system for image storage and processing, which has the functions of long-term image recording, voice recording, remote monitoring and control of images/voices.
Recording function: The video resolution is the core of the digital host because the video effect is more important to the Forensics than the on-site video effect. Most Digital Video Recorder can achieve on-site recording at 25 frames per second.
Password authorization function: in order to reduce the failure rate of the system and illegal entry, a password need to be set for procedures such as stopping video recording, arming and disarming the system and entering programming, so that unauthorized people cannot operate these procedures. The Password authorization is usually set as the multi-level password authorizations.
Work Schedule function: The user can program the working time for one certain period of time of one certain camera, which is also the unique function of the host of the Digital Video Recorder. The user can pre-schedule all the changes of holidays and working schedules into the program, which can realize the unattended in some extence.
All in all, the Digital Video Recorder represents the developing direction of CCTV monitoring system and it has been the first choice on the CCTV monitoring system market.
Digital Video Recorder, DVR Security System,, DVR, Digital Video Recorder H.264, DVR CCTV SHENZHEN SANAN TECHNOLOGY CO.,LTD , https://www.sanan-cctv.com
1.3.3.1 HRP substrate HRP catalyzes the oxidation of peroxide. The most representative peroxide is H2O2. The reaction formula is as follows: DH2+ H2O2 D+ H2O In the above formula, DH2 is the oxygen donor and H2O2 is the hydrogen acceptor. body.
The Digital Video Recorder is far superior to the analog monitoring equipment in terms of the image processing, image storage, retrieval, backup, network transmission, and remote control because of the adoption of the digital recording technology.
By the way, there are many BNC video interfaces on the back of the Digital Video Recorder, each interface can be connected to an analog suveillance camera. Just plug the interface, then it works, very convenient!
Generally speaking, the Digital Video Recorder can be divided into the hard disk video recorder, the PC type hard disk video recorder and the embedded hard disk video recorder, etc. Among them, the embedded hard disk video recorder does well in the stablity, the reliability, and the easy use, and the embedded hard disk video recorder will gradually invade the market of the PC type hard disk video recorder.
Besides, The main functions of the Digital Video Recorder include: monitoring function, recording function, playback function, alarm function, control function, network function, password authorization function and work schedule function.
Monitoring function: The monitoring function is one of the most important functions of the Digital Video Recorder. Whether it can monitor the on-site camera video clearly is a core issue of the surveillance system. Most Digital Video Recorders can achieve clear on-site surveillance.
Alarm function: The alarm function mainly includes the input alarm of the detector and the alarm of the image and video detection. Once received the alarm signal, the system will automatically start recording, and turn on the corresponding spotlight, siren and network output signal via the alarm output function. The image motion detection is the main alarm function of the Digital Video Recorder.
Control function: The control function refers to the omnidirectional control of the PTZ and lens via the host, which is generally finished by the dedicated decoder and the keyboard.
Network function: The user can do various operations to the host such as the monitoring, recording and control after the simple identification on the LAN or WAN, which is similar to the local opertaion.
Substrate classification of the kit enzyme