The functions of polymorphonuclear leukocyte (PMN) include adhesion, movement, phagocytosis, etc., which are important components of the body's natural immunity.

Reagents and materials

Staphylococcus aureus

2. Broth medium

3. Sexual beauty blue liquid

Method of operation

1. Preparation of bacterial solution Inoculate white staphylococcus aureus, and incubate in a 37 ° C incubator for about 12 h; heat in a water bath at 100 ° C for 10 min to kill the bacteria, and dilute to 6 × 10 ⁸ with sterile physiological saline.   Bacteria/ml spare;

2. Using a hemoglobin pipette to draw 40 μl of the earlobe or finger blood of the subject, immediately add the well of a clean concave slide containing 20 μl of heparin (concentration of 20 U/ml), gently stir and mix, and add the above staphylococcus Mix 20 μl of the solution thoroughly. Then placed in a covered container covered with wet gauze (this container is pre-warmed in a 37 ° C incubator), and applied in a 37 ° C incubator for 30 min, during which time it is shaken once every 10 min;

3. After the action is completed, take a small drop of the mixture on one end of the clean, oil-free slide and push it into a thin sheet. After being dried, it was fixed with methanol for 4 to 5 minutes, and stained with sputum blue solution for 2 to 3 minutes, and observed under an oil microscope. Randomly count 100 neutrophils, record the number of phagocytic and non-phagocytic leukocytes, and record the number of phagocytic bacteria for phagocytic leukocytes;

Result analysis

% of phagocytic cells = the number of cells phagocytized by bacteria in 100 neutrophils;

Phagocytic index = the total number of bacteria phagocytized by 100 neutrophils is divided by 100 to obtain the average number of phagocytic bacteria per leukocyte, which is the phagocytic index.

The functions of polymorphonuclear leukocyte (PMN) include adhesion, movement, phagocytosis, etc., which are important components of the body's natural immunity.

Reagents and materials

Staphylococcus aureus

2. Broth medium

3. Sexual beauty blue liquid

Method of operation

1. Preparation of bacterial solution Inoculate white staphylococcus aureus, and incubate in a 37 ° C incubator for about 12 h; heat in a water bath at 100 ° C for 10 min to kill the bacteria, and dilute to 6 × 10 ⁸ with sterile physiological saline.   Bacteria/ml spare;

2. Using a hemoglobin pipette to draw 40 μl of the earlobe or finger blood of the subject, immediately add the well of a clean concave slide containing 20 μl of heparin (concentration of 20 U/ml), gently stir and mix, and add the above staphylococcus Mix 20 μl of the solution thoroughly. Then placed in a covered container covered with wet gauze (this container is pre-warmed in a 37 ° C incubator), and applied in a 37 ° C incubator for 30 min, during which time it is shaken once every 10 min;

3. After the action is completed, take a small drop of the mixture on one end of the clean, oil-free slide and push it into a thin sheet. After being dried, it was fixed with methanol for 4 to 5 minutes, and stained with sputum blue solution for 2 to 3 minutes, and observed under an oil microscope. Randomly count 100 neutrophils, record the number of phagocytic and non-phagocytic leukocytes, and record the number of phagocytic bacteria for phagocytic leukocytes;

Result analysis

% of phagocytic cells = the number of cells phagocytized by bacteria in 100 neutrophils;

Phagocytic index = the total number of bacteria phagocytized by 100 neutrophils is divided by 100 to obtain the average number of phagocytic bacteria per leukocyte, which is the phagocytic index.

For more parameters and products, please refer to the website of Shanghai Shujun Instrument Equipment Co., Ltd.,