As the world's leading GM/knockout model animal commercial technology platform, after more than ten years of development, the game industry has served thousands of customers, with more than 1,300 academic citations. It is the most important and recognized genetic engineering model in the world. One of the animal business service platforms. Years of unremitting efforts in the genetic engineering model animal field, the game industry has accumulated rich experience in model animal preparation, and has been fully recognized by numerous customers.

In order to provide customers with faster and more efficient services, Saiye Bio has been committed to technological innovation. In the second half of 2015, Saiye Biotechnology applied the most advanced high-throughput electro-fertilization method to replace the method of manual injection of fertilized eggs, combined with the special optimization of superovulation technology and artificial intelligence (AI) AlphaKnockout gene targeting expert program system. The first batch of 100 CRISPR-AI knockout mice sperm cryopreservation projects were tested, and the data showed that the new technology greatly improved the success rate of the project.

Surprising test results allowed the Saiye creature to decide to pursue the victory. At the beginning of 2016, Saiye decided to launch the “ 10,000 Cases of CRISPR-AI Knockout Mouse Sperm Library ” project, which is known as the “Apollo Program”. Thanks to the accumulation of years of experience and the mature high-throughput carrier construction platform and electro-fertilization technology, Saiye Bio has established more than 3,800 CRISPR-AI knockout mouse sperm in just one and a half years. strain. It is expected that 10,000 CRISPR-AI knockout mouse sperm lines will be completed in 2018, and the sperm bank preparation for knockout rats has been on the agenda.

In the future, relying on “ 10,000 cases of CRISPR-AI knockout mouse sperm bank ”, the game industry is no longer limited to providing customized services for knockout mice, but expands the business segment to provide a complete human disease model for scientific research institutions and the pharmaceutical industry. Services such as seed conservation, production, supply and information consultation to promote the development of animal engineering in China's genetic engineering model at a wider, higher and higher level.

Introduction to innovative technology:

1. Tianhe No. 2 assists AlphaKnockout gene targeting expert system

The AlphaKnockout Gene Targeting Expert System developed by Saiye Biotechnology has realized the design of an intelligent knockout mouse program, and with the support of the powerful computing functions of the Tianhe No. 2 supercomputer, the design can be presented in 10 seconds ( ) ). The intelligent design process accelerated the progress of the tens of cases of CRISPR-AI knockout mouse sperm bank construction projects.

2. Optimized high-throughput electrical transfer technology

Traditional fertilized egg injection technology requires manual injection of eggs per microscopic operating system, and the operational efficiency has not been improved. Saiye Biological Joint Professional Equipment R&D unit has developed a system and supporting system suitable for the fertilization of mammalian fertilized eggs. The system can specifically transfect mouse fertilized eggs that have developed to a special stage and then transfect large quantities of fertilized eggs. The electro-rotation technology breaks through the efficiency bottleneck of the injection process, making high-throughput operation possible, thus greatly improving the efficiency of preparation.

3. Ultra-efficient fertilized egg acquisition technology

Studies have shown that the combined use of inhibin antiserum (IAS) and conventional superovulation hormone (PMSG and HCG) can significantly increase the number of superovulation in mice. Saiye Bio and the well-known bioreagent company jointly developed the ovulation induction reagent. After a series of optimizations, the number of eggs supplied to the oviposited mice was increased by 2-3 times, which greatly reduced the use of mice and increased animal welfare. It also accelerated the process of CRISPR-AI knockout sperm bank resuscitation.

4. New frozen storage technology system

Based on the original patented serum-free and non-programmed cryopreservation technology, Saiye Biotechnology has optimized a large number of systems for animal sperm and embryo cryopreservation technology, which has further improved recovery rate and birth rate, and has excellent stability. Sex. This provides stable cryopreservation support for the construction of our CRISPR-AI knockout mouse library.

Since its establishment 11 years ago, Saiye Biotechnology has been committed to technological innovation. We not only strive to improve the preparation efficiency of genetically engineered mice, but also actively explore and study ways to reduce the use of animals from the perspective of animal welfare. In addition, Saiye Biotechnology is also actively trying to use artificial intelligence technology and bioinformatics technology to promote the artificial intelligence process in the field of biotechnology. All these efforts have made Saiye Biotech become the world's leading commercial technology platform for GM/knockout mode animals, and become one of the most important and recognized genetic engineering model animal business service platforms in the world.

references:

1. T.Takeo and N.Nakagata, Superovulation Using the Combined Administration of Inhibin Antiserum and Equine Chorionic Gonadotropin Increases the Number of Ovulated Oocytes in C57BL/6 Female Mice. PLOS ONE on May 29,2015,10 (5)

2. Sean Chen, Benjamin Lee, Angus Yiu-Fai Lee, Andrew J. Modzelewski, Lin He. Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes .Journal of Biological Chemistry, 2016,291 (28):14457

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