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1 Scope This standard stipulates technical points for the cultivation and establishment of bay scallop seedlings.
This standard applies to bay scallop pro-shell selection, larval rearing, seed breeding, and cultivating.
2 Normative references The following documents contain provisions which, through reference in this standard, constitute provisions of this standard. For dated references, subsequent amendments (not including errata content) or revisions do not apply to this standard. However, parties that have reached an agreement based on this standard are encouraged to study whether the latest versions of these documents can be used. . For undated references, the latest version is applicable to this standard.
GB 11607 Fishery Water Quality Standard
SC/T 2005.2 Scallop oyster culture yield acceptance method
NY 5052 pollution-free food aquaculture water quality
NY 5062 Harmless Food Bay Scallop
NY 5071 Guidelines for the Use of Non-Hazardous Food and Fishery Drugs
3 Terms and Definitions The following terms and definitions apply to this standard.
3.1
Fullness
The percentage of wet weight in the soft body.
3.2
Specification qualification rate
The percentage of seed that meets specifications is the percentage of total seed.
3.3
Disability mortality rate
The number of broken shells and dead seedlings accounted for the percentage of the total number of seedlings.
3.4
Metamorphosis
The face plate of the larvae disappears and the secondary shells grow into the biological process of juveniles.
3.5
Seedlings of shell height 400μm-600μm
Shells with 400 μm to 600 μm shell height.
3.6
Commercial seedlings.
Shell height 3.0mm to 5.0mm juveniles.
4 Parents
4.1 shell height ≥5.Scm, wet weight ≥30g.
4.2 Quality
4.2.1 The senses should meet the requirements of Table 1.
4.2.2 The qualified rate and fullness of the specifications shall meet the requirements of Table 2.
4.3 Cultivation Technology
4.3.1 Cultivation pond cement pool or glass steel tank, 10m3 ~ 30m3, water depth 1.1m ~ 1.5m.
4.3.2 Water quality The water source complies with the provisions of GB 11607 and the cultivation water complies with the provisions of NY 5052.
4.3.3 The water temperature is gradually raised from the water temperature of the pro-beehive habitat to 18 to 20°C.
4.3.4 Salinity
25 to 31.
4.3.5 Lighting
500 lx to 1 000 1x.
4.3.6 Cultivation density
100/m3 to 120/m3, using multi-layer cages or single-layer floating cages as incubation containers.
4.3.7 Daily Management
4.3.7.1 Feed the bait with diatoms such as diatoms, golden algae or Platymonas or substitute feeds; feeds containing hormones or hormones are strictly prohibited.
4.3.7.2 Change the water daily 100% in the early and middle stages of the water change; change the water by one third every day in the late stage.
4.3.7.3 In the early and middle period of the inverted pool, the bottom of the pool shall be cleared once every other day; it shall not be poured before the egg is collected.
4.3.7.4 Absorb the bottom at the beginning and at the end of each day in the early and middle stages; increase the number of the bottoms properly in the late stage.
4.3.7.5 The temperature rises by 0.5°C to 1.0°C per day.
4.3.7.6 Inflatable continuous micro-inflation.
4.3.7.7 The medication and withdrawal periods are performed according to the provisions of NY 5071.
5 Larval breeding
5.1 Egg collection density ≤ 50 capsules/mL.
5.2 Fertilized sperm and eggs fertilize in the sea.
5.3 Incubation
5.3.1 Density
30 to 50 particles/mL.
5.3.2 Water quality Same as 4.3.2.
5.3.3 Water temperature
22°C to 24°C.
5.3.4 Salinity
25 to 31.
5.3.5 Removal of excess semen When the pro-scallops are removed, inflate and inflate the foam on the water surface (the foam contains a large amount of sperm) to remove excess semen and increase the hatching rate.
5.4 Cultivation Conditions
5.4.1 Water quality Same as 4.3.2.
5.4.2 Water temperature
22°C to 24°C.
5.4.3 Salinity
25 to 31.
5.4.4 Lighting
5001x or less.
5.4.5 Density
10/mL or so.
5.5 Daily Management
5.5.1 Feeding Fertilized eggs hatch to the D-shaped larval stage, that is, after 24 hours of fertilization, they can be fed with small-sized single-celled algae such as diatoms, golden algae, and Platymonas subtilis. The daily feeding amount was 2104 cells/mL; with the growth of larvae, the feeding amount of bait should be gradually increased, reaching 8104 cells/mL in the later period and feeding 6-8 times.
5.5.2 Changing the water Change the water twice a day, changing the water one third at a time.
5.5.3 The first inversion pool should be performed 25 to 30 hours after spawning, and the pool should be inverted every 3 days.
5.5.4 Absorb the bottom one at a time in the morning and evening.
5.5.5 Inflatable continuous micro-inflated with No. 100 or No. 120 bulk gas stones.
5.6 Collecting seedlings
5.6.1 When the larvae reach 30% or more of the time, the seedlings should be immediately poured and put on the seedlings. After placing the seedlings, the water temperature can be increased by 1°C to 2°C.
5.6.2 Kinds of seedlings such as polyethylene mesh or fine brown rope.
5.6.3 Treatment of the harvester Before using the polyethylene mesh, be sure to use 0.5% to 1.0% sodium hydroxide solution to soak and clean the oil stain; the mandrel must be repeatedly soaked, knocked, rinsed to remove debris, impurities and soluble harmful substance.
5.6.4 The amount of polyethylene seedlings put into the net is 2.0kg/m3 to 2.5kg/m3, and the diameter of the 3mm rope is 1 000m/m3 to 1500m/m3.
5.6.5 After the post-emergence management, the amount of water change shall be increased and the amount of aeration shall be appropriately increased after placing the seedling applicator. Check the condition of attachment and change the amount of feeding according to the number of seedlings attached.
5.6.6 Dosing and withdrawal periods are governed by the provisions of NY 5071.
5.7 seedlings
5.7.1 Specifications Shell height 400μm ~ 600μm.
5.7.2 Quality
5.7.2.1 The sensory size is uniform, the seeds are robust, and the full filament adhesion is strong.
5.7.2.2 The pass rate of specification pass rate, deformity rate and disability mortality rate is ≥ 95%; the total of deformity rate and disability mortality rate is ≤ 3%.
5.7.3 Inspection Rules
5.7.3.1 Before the sale of the seedlings before the sale, they shall pass the inspection.
5.7.3.2 Group Batch Rule and Sampling Count
5.7.3.2.1 The group batch rule is a batch of seedlings in a nursery pond.
5.7.3.2.2 Sampling counts Five to 12 meshes (or ropes) are drawn from different locations of a nursery and two are randomly cut from the upper, middle, and lower parts of the mesh (or rope). One or three buckles (or 5cm long rope) count the number of seedlings and calculate the number of seedlings on each mesh (or rope). Based on this, calculate the entire cultivation tank mesh (or brown rope). The total amount of seedlings on the pool.
5.7.3.2.3 Qualification rate of specification In the sampling and counting, the extracted seedlings of several samples shall be thoroughly mixed, and 50 to 100 seedlings (excluding disabled, dead and deformed individuals) shall be randomly selected and measured. Shell height, statistical specification pass rate.
5.7.3.2.4 Malformation rate and disability mortality At the time of sampling and counting, the extracted seedlings of several samples are fully mixed, and 200 to 500 seedlings are selected at random, and abnormalities and shell crushing are investigated. And the number of deaths, statistics deformity rate and disability mortality.
5.7.4 Decision Rules
5.7.4.1 One of the specification qualification rate, deformity rate and disability mortality rate, and sensory requirements does not meet the quality requirements, and it is determined that the seedlings in this batch are unqualified.
5.7.4.2 If there is any disagreement with the counting results, they can be negotiated between the purchaser and the seller and re-sampled and rechecked in accordance with the methods specified in this standard. The results of the re-inspection shall prevail.
6 cultivation of seedlings in the middle
6.1 Cultivation Technology
6.1.1 The site chooses shrimp ponds, reservoirs or calm inner bays.
6.1.2 Site disinfection shrimp ponds and reservoirs are disinfected with 500 mg/L quicklime or 30 mg/L to 50 mg/L bleach.
6.1.3 Water quality shall comply with NY 5052 regulations.
6.1.4 Water temperature
13 °C ~ 26 °C.
6.1.5 Salinity
25 to 33.
6.1.6 Transparency
60 cm to 80 cm.
6.1.7 Cultivation density
6.1.8 Daily Management
6.1.8.1 One week before the purchase of fertilizer and fertilizer, water is firstly applied to 50cm to 60cm. Apply inorganic fertilizer: nitrogen 3mg/L~5mg/L and phosphorus 0.3mg/L~0.5mg/L to inoculate artificially cultivated diatoms and golden algae. Or Platymonas algae feed.
6.1.8.2 After flushing the seedling bag seedlings pool for 3 days to 4 days, manually lift, place or gently swing to flush the floating mud on the seedling bags to maintain a good exchange of water inside and outside the bag and ensure good water quality and feed supply.
6.1.8.3 Sub-seedlings and Pouching With the growth of juveniles, seedlings and bags should be separated in time to evacuate the density.
6.1.8.4 Water quality control Water changes, fertilizers and water are used according to actual conditions to create conditions suitable for the growth and development of juveniles.
6.1.8.5 Growth and death observations Periodic observations of growth and death of juveniles and timely removal of dead shellfish.
6.1.8.6 Emergency Disposal When harmful red tides or oil spills occur in the adjoining or cultured sea areas, effective measures should be taken in time to avoid contamination of bay scallop seedlings.
6.2 Product seedlings
The 6.2.1 specification shell height is 3.0mm to 5.0mm.
6.2.2 Quality
6.2.2.1 The sensory size is uniform, the color is bright, the shell thickness is moderate, the shell edge is thin, and the vigor is strong (opening and closing of the shell in water).
6.2.2.2 The pass rate of specifications, deformity rate and disability mortality rate is ≥ 90%, and the deformity rate and disability mortality rate are 3%.
6.2.3 Inspection Rules
6.2.3.1 Pre-sale inspections The commodity seedlings should be tested before they are sold.
6.2.3.2 Group Batch Rules and Sampling
6.2.3.2.1 Group batch rules are sold as one batch for one delivery.
6.2.3.2.2 Sampling
6.2.3.2.2.1 Individual counting A device is randomly selected from the same seedling preservation or intermediate breeding apparatus as a sample and counted. Repeat the extraction 4 to 6 times, find the average number of commercial seedlings of the sample, and then calculate the total number of commercial seedlings according to the number of appliances.
6.2.3.2.2.2 Weight Count A batch of commodity seedlings shall be taken out from the seedling preservation or intermediate breeding apparatus. After removing the impurities, the total weight shall be weighed. Then 3 to 4 samples shall be randomly selected, weighed and counted. Each sample commodity seedling weight should be between 10g ~ 50g, find the average unit weight of the sample taken from the number of commercial seedlings, in accordance with the total weight of the total number of commercial seedlings.
6.2.3.2.2.3 Individual Count and Weight Count Relationships The two sampling count methods have the same effect.
6.2.3.2.2.4 Compliance rate In the sampling and counting, the commercially available seedlings of several samples are fully mixed, and 50 to 100 seedlings (excluding disabled, dead and deformed individuals) are randomly selected from the sample. High, statistical specification pass rate.
6.2.3.2.2.5 Malformation rate and disability mortality At the time of sampling and counting, the commercially available seedlings of several samples taken are thoroughly mixed and 200 to 500 commodity seedlings are randomly selected from them, and abnormalities, shell crushing and deaths are counted. , statistics of deformity rate and disability mortality.
6.2.4 Decision Rules
6.2.4.1 One of the specification qualification rate, deformity rate and disability mortality rate, and sensory requirements did not meet the quality requirements, and it was determined that the batch of commodity seedlings was unqualified.
6.2.4.2 If there is any objection to the counting result, it may be negotiated between the purchaser and the seller, and the re-sampling shall be re-sampled in accordance with the methods specified in this standard, and the result of the re-inspection shall prevail.
7 Intermediate cultivation of commodity seedlings
7.1 Cultivation Technology
7.1.1 The site selects a sea area where the water flow is gentle, there are no large waves, the food is rich, or sea area where scallops are developed.
7.1.2 The water quality shall comply with the provisions of NY 5052.
7.1.3 Density
7.1.3.1 Mesh bag method Each bag contains 300 to 500 capsules. Each string is hung with 10 bags. A 60m floating raft can hang 100 strings to 120 strings.
7.1.3.2 The cage method puts 300 to 500 capsules per layer. A 60m floating raft can hang 100 cages.
7.1.4 The seedlings of the seedlings shall be firstly reared on the sea for 3 days to 5 days for adaptation and recovery, and then divided into seedlings to 18- or 16-mesh bags for continued raising. After about 15 days, when the shell height reaches 10 mm or more, It is raised in a holding cage with a mesh of 8mm to 10mm.
7.1.5 Emergency Disposal When incidents such as red tides or oil spills occur in the adjacent or aquaculture sea areas, effective measures shall be taken in time to avoid contamination of bay scallop seedlings.
7.2 Seedlings
7.2.1 Specification shell height ≥2.0cm.
7.2.2 Quality
7.2.2.1 The sensory size is uniform, the color is bright, the shell thickness is moderate, the shell edge is thin, and the activity is strong (the shell is open and closed in the water).
7.2.2.2 The qualification rate, deformity rate and disability mortality rate of the specification are ≥ 90%; the total deformity rate and disability mortality rate are ≤ 5%.
7.2.3 Inspection Rules
7.2.3.1 Before inspection, the test seed must be passed before the sale.
7.2.3.2 Group Batch Rules and Sampling
7.2.3.2.1 Group batch rules are sold as one batch for one delivery.
7.2.3.2.2 Sampling
7.2.3.2.2.1 Individual counting A device is randomly selected from the same nursery or intermediate breeding apparatus as a sample and counted. Repeat the extraction 4 to 6 times, find the average number of seed samples, and then calculate the total number of seedlings according to the number of instruments.
7.2.3.2.2.2 Weight Count All batches of seedlings are to be removed from the seedling or intermediate cultivating apparatus. The total weight is weighed after removal of impurities. Then 3 to 4 samples are randomly selected, weighed, and counted. The weight of each seed sample should be between 10g and 100g. Seek the average number of seeds per unit weight of the sample. Calculate the total number of seeds in this batch according to the total weight.
7.2.3.2.2.3 Individual Count and Weight Count Relationships The two sampling count methods have the same effect.
7.2.3.2.2.4 Rule qualification rate When sampling and counting, the seed samples of several samples taken shall be thoroughly mixed, and 50 to 100 seedlings (excluding disabled, dead and deformed individuals) shall be selected at random, and the shells shall be measured. High, statistical specification pass rate.
7.2.3.2.2.5 Malformation rate and disability mortality At the time of sampling and counting, the seedlings of several samples taken are thoroughly mixed and 100 to 200 seedlings are selected at random, and the number of malformations, shell crushing, and deaths are counted. , statistics of deformity rate and disability mortality.
7.2.4 Decision Rules
7.2.4.1 One of the specification qualification rate, deformity rate and disability mortality rate, and sensory requirements does not meet the quality requirements and it is determined that the seedlings in this batch are unqualified.
7.2.4.2 If there is any objection to the counting results, it may be negotiated between the purchaser and the seller, and the re-sampling shall be re-sampled in accordance with the methods specified in this standard, and the result of the re-inspection shall prevail.
8 Develop
8.1 Shallow sea bream culture
8.1.1 The environmental conditions should meet the requirements of Table 4.
8.1.2 Shallow sea breeding facilities consist of floating rafts, floats, fixed rafts, rafts, and cages. Do not use toxic materials.
8.1.3 The establishment of aquaculture facilities shall be divided into sea areas and the locations shall be determined, and the waterways shall be reserved. The direction of the rows shall be perpendicular to the flow direction, and the spacing shall be 10m to 20m.
The cage spacing is 0.5m to 0.7m, and a 60m floating cage can hang 80 cages to 100 cages.
8.1.4 The uppermost layer of cultured aquaculture cages is from 1 m to 2 m above the water surface.
8.1.5 Cultivation density: 7104 grains to 10104 grains per hectare of water surface (including vacant water area for waterways, etc.); breeding cages with a diameter of 30cm per layer, 25 to 35 grains.
8.1.6 Daily Management
8.1.6.1 Remove all predator organisms and attachments, timely scrub and remove the hostile organisms, find out the spawning and attachment time of barnacles, oysters, etc., and the vertical distribution and plane distribution of larvae in the sea area of ​​the seedlings, and try to avoid barnacles. During the peak period of oyster attachment, production operations such as sub-bags and cages are performed.
8.1.6.2 During the seasons in which a large number of attachments to cultured aquifers are adjusted, the water layer should be properly lowered; before the arrival of large wind waves, the entire trusses should be lowered to reduce losses. With the growth of scallops and weight gain, floats should be added in time to prevent sinking of the trusses and keep the floats sinking to the surface of the water without sinking.
8.1.6.3 Emergency Disposal When incidents such as red tides or oil spills occur in contiguous or aquaculture sea areas, effective measures should be taken in time to avoid contamination of bay scallops. If the bay scallops have been contaminated, they should be destroyed on the spot and are strictly prohibited from listing.
8.2 Pond bottom sowing
8.2.1 The environmental conditions shall meet the requirements of Table 5.
8.2.2 Cultivation density: 10104 grains to 15104 grains per hectare of water.
8.2.3 Daily Management
8.2.3.1 Fertilizer and Feeding Use inorganic fertilizers and fertilizers to inoculate artificially cultivated monophyletic baits such as diatoms, golden algae or Platymonas, or substitute feeds; feeds containing substitute hormones or hormones are strictly prohibited.
8.2.3.2 Clean production shall be maintained for more than 15 days in a well-characterized sea area until it reaches the pollution-free food quality requirement.
9 Calculation of aquaculture area Shallow sea culture is carried out in accordance with the provisions of SC/T 2005.2.
10 Calculation of aquaculture production Shallow sea aquaculture is carried out in accordance with the provisions of SC/T 2005.2.
11 The quality of cultured products is determined in accordance with NY 5062.
Pollution-free Food - Bay Scallop Breeding Technical Specifications
Published on 2001-09-03 2001-10-01 Implemented by the Ministry of Agriculture of the People's Republic of China