1. Ensure the pure culture of the strains, be careful not to use strains contaminated by other bacteria, and do not connect and cultivate them at close range.
2. Strictly control the number of passages of the bacteria, reduce mechanical damage, and ensure the vigor of the bacteria.
3. Appropriate low-temperature storage of strains. Low-temperature strains such as mushrooms and shiitake mushrooms are beneficial to preserve the vigor of mycelium at 4°C, and high-temperature strains such as straw mushrooms are beneficial to preserve the vitality of mycelium at 16°C. .
4. Avoid multiple passages in a single or the same medium; strains should not be used for too long, as over-age strains will age, and aging and degradation are organically linked, and strains with weak viability are prone to degradation.
5. Strains should be rejuvenated on a regular basis, under conditions of suitable temperature, suitable pH, sufficient oxygen, and free of bacteria.
6. Perform spore separation every year to discover excellent strains through sexual reproduction, and consolidate the genetic characteristics of excellent strains through tissue separation.

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