Nucleic Acid (DNA/RNA) Extraction Kit
1. Introduction Nucleic Acid Extraction Reagent Kit,Nucleic Acid Extraction Kit,Covid-19 Nucleic Acid Extraction Reagents,Nucleic Acid Test Kits Jilin Sinoscience Technology Co. LTD , https://www.jlgkscience.com
The total viral nucleic acid extraction kit is suitable for extracting total viral nucleic acid from serum, plasma, tissue homogenate and other samples. The kit is based on silica column purification technology, which eliminates the need for toxic phenol-chloroform extraction and time-consuming alcohol precipitation. This product has successfully extracted nucleic acids from hepatitis B A/C, hepatitis C, and norovirus standard. The obtained DNA/RNA can be directly used in a series of downstream experiments such as PCR, RT-PCR, and LAMP.
Notice:
1. The carrier RNA solid must be dissolved in Nuclease Free Water to 1µg/µl before use, and vortex to dissolve. Store in aliquots at -70°C. If you need to store it at -20℃ for a long time, please repackage it according to the number of times of use.
2. Dissolve Proteinase K (20mg/ml): Add Proteinase Dissolve Buffer to dissolve Proteinase K to a final concentration of 20mg/ml. Proteinase K dry powder can be stored at 2-8°C for one year, but dissolved Proteinase K must be stored in aliquots at -20°C. Repeated freezing and thawing of Proteinase K can affect its activity.
3. Buffer VHB must be diluted with 14 ml absolute ethanol before use and stored at room temperature.
4. Buffer RW2 must be diluted with 80 ml of absolute ethanol before use and stored at room temperature.
3. Shelf life
Except for Proteinase K and Carrier RNA, other components of this product can be stored at room temperature (15-25°C) for 12 months, and should be stored at 2-8°C for long-term storage. Proteinase K and Carrier RNA dry powder are transported at room temperature. Please store at -20°C after receiving the test product, and store at -20°C after dissolving.
Honey bergamot processing
The honey bergamot is one of the Sichuan candied fruits and has the effect of promoting the circulation of gas. The processing technology is as follows: 1. Raw material processing: The fruit of the complete shape and the same size of the nine mature bergamot are used as raw materials. The raw materials are washed and cut into thin slices of about 1.5 cm in thickness. If the bergamot is too large, it must first be cut in half and cut into slices. 2, gray drift: Preparation of 2 ~ 3% lime water solution, the raw materials will be placed in lime water soak 8 to 10 hours, when the color of bergamot tablets from white to yellow, you can remove. 3, water drift: the bergamot tablets rinsed in water, so that the color of bergamot tablets can be removed. 4. Blanching: Put the bergamot slices in a boiling water pot and cook for 3 to 4 minutes. And completely remove the lime smell, drain water and wait. 5, preparation of sugar; raw material and sugar ratio of 1:0.7, the sugar is formulated as 40% sugar, and add 0.2% citric acid in the sugar, 0.5% salt, 0.05% potassium sorbate, the bergamot tablets Put it in sugar and cook it together. 6, translucent sugar: can be used once boiled through the sugar, bergamot tablets and sugar cook together, the beginning of the heat can be large, and constantly stirring, with the cooking time is extended, the water continues to evaporate, the sugar is constantly growing Buddha tablets are also Gradually absorb sugar, when the bergamot becomes transparent, the sugar concentration reaches more than 60%, when the fire is slow, or caramelization occurs, the product color will be dark brown until black. When the final sugar liquid is in the form of a thick gel, it cannot be reheated. Take the bergamot flakes out of the sugar liquid. 7. Drying: Dry at 65 degrees Celsius for 16 hours, so that the water content is 23 to 25%. 8, packaging: composite film pouch packaging. 9, finished product flavor: sweet and sour and slightly salty, crisp. In the processing can be artificially stained yellow, blue, green and other colors.