The utilization of plant growth promoting rhizobacteria (PGPR) in agriculture is continuously increasing as it offers an effective tool to replace the use of chemical fertilizers, pesticides and other harmful supplements (Ansari et al., 2017, Ansari and Mahmood, 2019a). Growth promoting substances are produced in huge quantities by the action of these rhizosphere microorganisms that directly or indirectly influence the overall morphology and physiology of the crops. Recent advances in the field of sustainable development relies on the use and diversity of PGPR, their colonizing capability and the mechanism of action that may be used to facilitate their application as a dependable element in the management of sustainable agricultural system. Azotobacter is a group of Gram negative, free-living, nitrogen fixing aerobic bacteria inhabiting in the soil. They are oval or spherical in shape and form thick-walled cysts (dormant cells resistant to deleterious conditions) under unfavorable environmental conditions. Around six species in the genus Azotobacter have been reported, some of which are motile by means of peritrichous flagella while others are immotile . They are typically polymorphic having size ranging from 2 to 10 µm long and 1 to 2 µm wide. The genus Azotobacter was recognized in 1901 by Dutch microbiologist, botanist and founder of environmental microbiology-Beijerinck and his co-workers as the first aerobic free-living nitrogen fixer. These bacteria are known to exploit atmospheric nitrogen for their cellular protein synthesis which is mineralized in the soil, imparting the crop plants a considerable part of nitrogen available from the soil source. Azotobacter spp. is sensitive to acidic pH, high salt concentration and temperature . They pose advantageous impacts on the crop growth and yield through the biosynthesis of biologically active substances, instigation of rhizospheric microbes, production of phytopathogenic inhibitors, alteration of nutrient uptake and eventually magnifying the biological nitrogen fixation . Research on Azotobacter chroococcum in crop production has shown its importance in improving plant nutrition and amelioration of soil fertility . Several strains of Azotobacter are found to be able to produce amino acids when grown in culture media supplemented with various carbon and nitrogen sources . Such substances produced by these rhizobacteria are implicated in several processes thus leading to plant-grown promotion . The scope of utilizing Azotobacter chroococcum in research experiments as microbial inoculant through release of growth substances and their impact on the plant has markedly improved crop production in agriculture. Azotobacter vinelandii Biodep Biotechnology Co. ,Ltd. , https://www.biodep.com
Hairy rabbits produce more than eight hairs
First, improve the feeding environment. The appropriate ambient temperature for the long-haired rabbits is 5°C to 25°C. When the temperature exceeds 30°C, the feed intake decreases, the wool production decreases, and nutrient consumption below 0°C also affects the amount of wool produced. The rabbit house should be insulated in winter, summer heatstroke, clean and fresh air. Second, strengthen the rearing management after shearing. On the 2nd day after hair-cutting, each rabbit was intramuscularly injected with 0.1g of vitamin B12, and the hair production cycle could be shortened by 10 days, or by intramuscular injection of 1 ml of fattening spirit and 0.07 g of vitamin B12. Third, add trace element additives. Each adult rabbit was fed with 10 mg of trace element additive every day. Feeding for 4 months continuously increased the hair production by 27%. Fourth, feed leeks and soybeans. Before picking the hair, feeding them with leek and soybeans will increase the coat and make it look lighter. Five, feeding. Each female rabbit is fed with 7 grams of lotus root powder each day, and the amount of wool produced can be increased by 10%. Six, medicine bath. Calculated for every 20 long-haired rabbits, 100 grams of Euphorbia militaris, 100 grams of Sophora flavescens, and 2.5 kilograms of water were added. After decocting, the filter juice was added to the residue. Then 100 grams of sulphur powder and 5 kilograms of boiling water were added to stir. . Seven, with wine, ginger reminder. Mix the rabbit body with 1 tablespoon of liquor and a little ginger juice, and make the new long rabbit hair dense and homogeneous. Eight, use raw eggs to urge hair. Eggs are rich in sulphur-containing amino acids and have a long hairy effect. Every 5 to 10 long-haired rabbits should feed one egg each day in the feed.