Anaerobic incubator carbon dioxide tissue cell culture operation precautions

Anaerobic incubator carbon dioxide tissue cell culture one-button operation

Anaerobic incubator use

(1) The anaerobic environment of the operation room is formed:

  1. 1. Place the necessary accessories and utensils as required;
  2. 2. Turn on the light through the power supply, turn on the temperature controller, and adjust the required temperature;

3. Place 1000g palladium pellets (closed) and 500g desiccant in the operating room and put in the Meilan indicator paper (closed);

4. Close the sampling indoor and outdoor doors and vacuum check;

5. The first replacement in the operating room (nitrogen replacement):

A. Put the latex gloves on the viewing plate flange and tighten

B. Turn on the nitrogen inlet, open the nitrogen control valve 1, bulge the glove, close the valve 1, and then tighten the bag;

6. The second replacement of the operating room is repeated for one nitrogen filling process. The sampling chamber is first evacuated. Be careful to open and close the exhaust with the foot switch at any time. This was followed by three consecutive nitrogen substitutions.

7. The fourth replacement of the operating room (mixed gas replacement): (mixed gas ratio is N2 85%, H2 5%, CO10%)

A. Change the air circuit to open the air intake valve 3 intake air, the sampling chamber first draws a vacuum when inflating, and open and close the exhaust gas with the foot switch at any time;

B. Turn off the mixed gas valve 3, and open the valve 5, so that the mixed gas passes through the flow meter, adjust the flow meter, and the flow rate is about 10 ml per minute;

C. The mixed gas is repeated 2-3 times, and after the above process, an anaerobic environment is basically formed.

8. Open the deoxidizing agent in the operating room, turn on the deoxygenation catalyst power supply to carry out catalytic deaeration, open the Meilan indicator paper to observe the change after one hour, and do not change the color to the anaerobic environment in the operation room;

9. Turn on the UV lamp and clean it indoors. The time is determined according to the specific experiment.

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