Dental Vacuum Former,Vacuum Forming Machine,Vacuum Forming Equipment,Automatic Vacuum Forming Machine Hebi Tianchi Trading Co., Ltd , https://www.hebitianchi.comr + Quit button to turn it off. If no operation is performed within 10 minutes, the instrument will automatically shut down.
Glycohemoglobin standard operating procedure
NycoCard Reader II glycated hemoglobin meter SOP
First, the instrument
Step 1: Remove the cap from the pen and insert it into the pen holder.
Step 2: Press The ON/Select button is turned on, and the screen displays “Adjusting...â€. Do not touch the pen during this process. A beep sounds indicating that the adjustment is correct, then “Calibrate†appears; two beeps indicate an adjustment error and “Adjust error†is displayed. This process takes about 30 seconds, please wait.
The third step: press Enter Key, when the message “Place pen...†is displayed, remove the pen from the jack, place it on the whiteboard for correction, and make a beep and the word “Enter test†appears to indicate that the correction is complete.
Step 4: Press the Enter key and “Test: D-Dimer New†will appear. [At this time, pressing the Select button will display items such as "CRP wholeblood", "CRP serum/plasma", and "HbA1c (glycated hemoglobin)". ]
Step 5: After selecting the detected item, press the Enter key to test.
Step 6: After the test is completed, press the Ente
Second, the reagent
Glycosylated hemoglobin SOP Step 1: Precipitate hemoglobin: Add 5 μl of whole blood to a centrifuge tube containing R 1 / reagent and mix. Allow to stand for 2 minutes, up to 3 minutes.
Step 2: Add the sample: mix again to obtain a uniform suspension. Accurately add 25 μl of the suspension to the test well with a pipette and allow the reaction mixture to fully wet the film for about 10 seconds.
The third step: adding R2 / Wash solution: add 25μl R 2 / washings were added to the reaction hole. Allow the lotion to fully wet the film for 10 seconds.
Step 4: Reading: Within 5 minutes, read the result using the reader.
Reference value: 4.5% - 6.3%
D- dimer SOP
Step 1: Pre-cleaning: Pipette 50 μl of R 2 / washing solution into the reaction well of TD / test card, and let the washing liquid fully infiltrate the film in the well. Be careful not to touch the reaction membrane.
Step 2: Add sample: Add 50 μl of citrate anticoagulated platelet-free plasma sample control to the well. The sample should penetrate into the well within 50 seconds.
Step 3: Add R 1 / coupling solution: Add 50 μl of R 1 / coupling solution to the reaction well. Mix the coupling solution bottle twice and mix it before adding. The coupling solution should penetrate into the card within 50 seconds.
Step 4: Washing : Add 50 μl of R2 washing solution to the well of the test card.
Step 5: Reading : When the washing liquid is completely absorbed by the film, read the result with a reader within 2 minutes.
Intra- assay quality control : Positive control is used to confirm the validity of the kit and the correctness of the experimental operation. The measured value should be within the bottle labeling range.
Note : 1. The sample should be placed vertically 1 cm above the film. 2. Try to avoid bubbles in the capillary and avoid residual specimens outside the capillary.
3, to avoid bubbles on the film, do not touch the film with the sample head.
Reference value: < 0.3mg/l
C- reactive protein SOP
Step 1: Sample dilution : Add the sample or positive control to a 5μl glass capillary and put the tube into the tube.
Step 2: R 1 / dilution in the centrifuge tube ; also accurately transfer 5 μl of sample or positive control, add the centrifuge tube containing R 1 / dilution, cover and mix thoroughly for 10 seconds.
Step 3: Add sample: Add 50 μl of diluted sample or positive control to the TD / test card hole and allow the sample to fully wet the film for about 30 seconds.
Step 4: Add R 2 / hydration: Add a drop of R 2 to the well and allow it to fully wet the film for about 30 seconds.
Note: The vial should be placed vertically 1 cm above the film.
Step 5: Add R 3 / Wash Solution: Add a drop of R 3 to the well and allow it to fully wet the film for about 20 seconds.
Step 6: Reading: Within 5 minutes, read the result using the reader.
Reference value: < 6mg/l
Urinary microalbumin SOP
Step 1: Sample dilution: Add 50 μl of urine sample or control to a centrifuge tube containing R 1 /diluent and mix well.
Step 2: Adding: Add 50 μl of diluted sample or control to the TD / test card hole and let the sample fully wet the film for about 50 seconds.
Step 3: Add R2/ coupling solution: Add 50 μl of R 2 / coupling solution to the well and allow the reagent to fully wet the membrane for about 50 seconds.
Step 4: Add R3/ washing solution: Add 50 μl of R 3 / washing solution to the reaction well and allow it to fully wet the film for about 50 seconds.
Step 5 : Reading: Within 5 minutes of the reaction, read the result using a reader.
Reference value: < 20mg/l