Application of ultracentrifuge in public experimental platform

The study of systems biology is the frontier research direction of Zui, which is not the function of a specific gene, protein or cell. It focuses on the study of genome, proteome and cell biology as a whole. To study their interactions in life state and specific conditions, so as to further understand life itself, reveal the occurrence and development of diseases, and provide more accurate information and technology platform for the development of new drugs. In the whole process of system biology research, centrifugation technology is “ubiquitous”, and ultracentrifuges are used as separation tools in nucleic acids, proteins and cells. In addition, ultracentrifuges can be used as an analytical tool to determine the structure of proteins, the interactions between proteins and proteins or small molecules, and molecular weight. It can also help scientists further study different aspects of proteins.

Ultracentrifuge is an indispensable technical means of biochemistry and molecular biology. Widely used in the separation and purification of macromolecules, cells, organelles, etc. Some information about cells, organelles, viruses, and biological macromolecules can be directly obtained by centrifugation or centrifugation, and some provide a basis for further chemical analysis, biological function measurement, and morphological observation of ultrastructure. Different methods and techniques can be used depending on the purpose. The solution can be divided into supernatant and sediment by ultracentrifugation, but the obtained precipitate and the supernatant are not uniform. The former contains all the particles in the initial solution of zui, while the latter still contains a small amount of particles, but the size is smaller than the precipitated particles. To be much smaller, the supernatant thus obtained can be further separated by higher speed centrifugation, called differential centrifugation. If you need to get a pure substance, you should use analytical over-dission technology. Density ultracentrifugation can be used to understand certain physical properties of a substance, such as sedimentation coefficient and approximate density, and to obtain a certain amount of relatively pure material.

The rotor is also the core of the centrifugal technology. The centrifuge is used to separate the samples. In addition to the centrifuge, the rotor is also indispensable. To achieve good separation, the choice of rotor is very important. The purity after separation of the sample and the time of separation have a great relationship with the type of rotor. Our public experimental platform has two kinds of rotors, namely fixed angle rotor and horizontal rotor.

The purpose of building a public experimental platform With the strong development of the college, the contradiction between scientific research and teaching resources has become increasingly prominent. After investigation, research and discussion, the college believes that an international pharmaceutical college should have an open public experimental platform. The "985" project was used to centralize the construction of a multidisciplinary shared experimental platform to achieve hardware resource sharing. It aims to build a public technology platform for schools and scientific research institutions, provide open public services, reduce the repeated purchase and idleness of experimental instruments and equipment, and save state funds. Establishing an open, research, and comprehensive professional experimental platform is more conducive to cultivating students' ability to comprehensively analyze and solve problems. The experimental platform is managed by the college, and the instruments and equipment of the public experimental platform are centrally managed, unified use, and standardized operations. Teachers of different fields are also assigned professors to guide the work. The total experimental area of ​​the Pharmacy School's public experimental platform is more than 600 m2. The school's "985 Project" has invested nearly 12 million yuan to build a public experimental platform with a certain scale, and has more than 20 large-scale precious precision instruments and equipment. Typical test instruments include ultracentrifuge, LC/MS, capillary electrochromatography analyzer, preparative and analytical high performance liquid chromatography, protein purification instrument, flow cytometry and other types of test instruments. The trial run has achieved good results.

Management method of ultracentrifuge in public experimental platform Ultracentrifuge in addition to normal teaching tasks in the public experimental platform, but also provide testing and analysis services for other scientific research units and enterprises. At present, the ultra-centrifuge provides fast, high-quality and thoughtful service and strong support for the research work of our institute, the School of Chemical Engineering, the College of Life Sciences, the School of Medicine, the College of Agriculture, the Shanghai Institute of Pharmaceutical Industry and the Shanghai Branch of the Chinese Academy of Sciences. The instrument is well received by teachers and students in the public experimental platform.

The ultracentrifuge is open to all teachers and students and social researchers. After the training, the user can do it himself. If the equipment is damaged due to illegal operation, it shall be liable for compensation. In order to obtain the qualifications for hands-on operation of the instrument, the user must participate in the induction training of the public experimental platform equipment and pass the open-book examination before obtaining the self-operating license. The public experimental platform often organizes free instrument training as needed. Teachers and students can register themselves and participate in relevant training according to their own needs. The use of ultracentrifuges must first pass the safety and instrumentation training of the public experimental platform, and then reserve the experiment. The public experimental platform is open 24/7. The public experimental platform also opened the core course "Experimental Technology and Instrumental Analysis" for engineering graduates of our school. More than 100 students enthusiastically signed up for training to further promote the cultivation of innovative talents, which played a very good role. We will continue to explore and improve the open management model of the public experimental platform.

The operation efficiency of the ultracentrifuge has proved that the open public experimental platform and the open experimental platform management system established by the College of Pharmacy solve the problems of “special management sharing”, “resource sharing” and “repetitive purchase” through schools and colleges. Concentrated investment makes the limited funds play a big benefit. After a period of running-in, this reform has achieved good results.

The ultracentrifuge has maintained a high usage rate since 2006, and the number of scientific research machines per year is more than 2,000 hours per unit. The number of test samples is about 10,000 and nearly 6,000 hours of experimental teaching tasks. The public experimental platform Realizing the true integration of teaching and scientific research innovation, it also provides a new opportunity for the development of public experimental platforms.

Abamectin (Avermectins)INDICATIONS
For the treatment of livestock and poultry nematodes, acariasis and other parasitic insect disease.
DOSAGE AND ADMINISTRATION
Pouring or rubbing.
Cattle, pigs, dogs and rabbits: 0.1ml/kg body weight for a a single dose.
The formulation should be applied along the mid-line of the back in a narrow strip between the withers and tailhead for cattle and pigs. Rubbed the back of the inside of the ear for dogs and rabbits.
ADVERSE REACTION
In horses, adverse reactions may include itching and edema. In poultry, may include death, lethargy or loss of appetite.
PRECAUTIONS
Use cautiously in animals sensitive to Avermectin. Strictly follow the storage condition. Toxic to aquatic life.

Avermectin

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