The preservation of edible fungus strains can effectively maintain its excellent breeding properties and prolong the useful life of good varieties. Therefore, it is of equal importance to the breeding of new varieties and is an indispensable important link in the production of edible fungi. However, regular and strict storage methods require some equipment and have high requirements on operating technology. For the majority of mushroom farmers, some simple soil methods are more feasible and more popular.

The test tube mother seed to be stored at the bottom of the well is stored at low temperature, sealed with paraffin with a sterile rubber stopper, sealed in a closed jar or plastic bag, and sunk into the bottom of the well for storage for 2 to 5 months.

Paraffin sealed storage Cut the wax paper into 6-8 cm squares and soak in 75% alcohol for 1 minute. Rotate and sterilize the tube mouth of the cultured test tube above the flame of the alcohol lamp, remove the wax paper with tweezers, and tighten the tube mouth. Then immerse the mouth of the wrapped wax paper in the melted paraffin, and completely immerse the wax paper so that the wax paper is tightly sealed on the wall of the tube without leaking air. After the paraffin has cooled and solidified, store it in the refrigerator or at room temperature.

Cellophane sealed storage 15 g of gelatin (adhesive), 2 g of copper sulfate, 1-2 drops of beautiful blue (indicator), dissolved in 100 ml of hot water, formulated as a sealing agent. Unplug the newly overgrown female seed with a cotton plug, with the mouth facing downward, dipped in a sealing agent, paste cellophane soaked in 95% alcohol, and melt seal with paraffin. At room temperature, the bacteria can be stored for at least 8 months, and can prevent the infestation of mold or mite. Injecting sterile mineral oil (liquid paraffin) on the slope can greatly extend the storage time.

Manure storage This method is suitable for storing rot fungi such as Agaricus bisporus. Dried and broken compost such as decomposed cow dung, dried, 300 grams of dry compost, sieved with a 5 mm pore size sieve, added 1000 ml of water to humidity, filled into a test tube, occupying a tube depth of 1/3 to 1/2, at 0.15 Sterilize under MPa for 2 hours. After confirming the sterility, inoculate, after the mycelium is full, store it in a refrigerator at 3 ° C, and transplant it every 2 years. In long-term storage, if the culture medium is dehydrated, the stored bacteria should be inoculated on the agar slope in time to activate it, and then continue to be stored. After 4 years of continuous storage in this method, its production characteristics did not change significantly.

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