RT-PCR Test Reagent Kit Of COVID-19(ORF1ab, N, E)
This RT-PCR Test Reagent Kit Of COVID-19(ORF1ab, N, E) is a real-time, RT-PCR test intended for the qualitative detection of nucleic acid from SARS-CoV-2 in oropharyngeal and nasopharyngeal swab specimens from individuals suspected of COVID-19 by their healthcare provider.
This test is also for use with anterior nasal swab specimens that are collected using the COVID-19 Test Home Collection Kit when used consistent with its authorization.
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Exchange resin requirements. Use sulfonated polyethylene resin. The resin must not be degraded at 100°C, not hydrolyzed under moderate acidity conditions, and the resin can be used for a long time.
Extraction conditions. The pH value is 1.2～1.3, the temperature is 80℃, and the time is 1 hour.
******precipitation. The concentrated pectin is cooled to room temperature, and solid pectin is obtained by precipitation. ****** The coagulation of pectin must have electrolyte to promote its complete precipitation.
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Citrus peel extraction pectin method
Pretreatment of citrus peel. Dried citrus peels are rehydrated first to remove sugars, acids, pigments and some bitter substances that affect the quality and flavor of pectin. Soak in warm water at 50°C and rinse for 2-3 hours to prevent the hydrolysis of pectin by pectinase. Put the soaked citrus peel in boiling water for 3 to 5 minutes to prevent loss of pectin.